Another strategy, presented by AthenaDAO at Vitalia:
Related:
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medaura
#164
Thank you, all’s well so far, I was nervous re: abnormalities given mg crazy stack at the time but all looks good so far! Now I’m taking choline, fish oil, hyaluronic acid, prebiotics, and occasionally collagen powder on top of my prenatals.
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medaura
#165
Yes but this requires a time machine as well for 99% of us to be relevant. Perhaps for our daughters it might become an option though.
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lurexlu
#166
Great! And I hear you… I’m v nervous too (in advance). Which powder is the cleanest? Dr Rhonda Patrick said she stayed away even from collagen during pregnancy to avoid any impurities.
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medaura
#167
I honestly didn’t go down the rabbit hole of researching the cleanest powder. Got the one with the best reviews but don’t take it daily because I don’t love the taste it creates with milk. I figure though there’s no point in getting anal retentive with every source of possible contamination. Sadly there’s guaranteed to be microplastics in the placenta and breast milk — what am I to do? There’s inadvertent exposure to things we don’t want happening all around us. I just try to eat well and I use a reverse osmosis water filter too and leave the rest to chance.
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A new paper out of McGill University in Montreal (actually the university that led the expedition to Easter Island that discovered Rapamycin).
several unproven IVF ‘accessories’ have emerged including so-called ovarian rejuvenation which entails placing fresh autologous platelet-rich plasma (PRP) directly into ovarian tissue. Among cellular responses attributed to this intervention are reduced oxidative stress, slowed apoptosis and improved metabolism. Besides having an impact on the existing follicle pool, platelet growth factors might also facilitate de novo oocyte recruitment by specified gene upregulation targeting uncommitted ovarian stem cells.
this proposal is the first to discuss intraovarian platelet cytokines followed by low-dose, phased rapamycin. For refractory cases, this investigational, tailored approach could amplify or sustain ovarian capacity sufficient to permit retrieval of competent oocytes via distinct but complementary pathways—thus reducing dependency on oocyte donation.
Given that intraovarian injection of PLT growth factors can increase serum AMH (indicating expansion of the follicle/oocyte unit) [7,77] and mTOR inhibition has been suggested to boost ovarian reserve [78], a bespoke protocol incorporating both might provide a useful synergy.
Open Access Paper:
相关：
Rapamycin improves the quality and developmental competence of in vitro matured oocytes in aged mice and humans
After intracytoplasmic sperm injection (ICSI) and further culture of human oocytes, the high-quality embryo rate in the rapamycin group was significantly elevated. Overall, rapamycin improved IVM outcomes of oocytes from aged mice and older women. The specific mechanism of the positive effects of rapamycin on IVM outcomes might be reducing ROS levels, mitigating DNA damage, and promoting developmental potential.
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A new story about the rapamycin / menopause study at Columbia University:
Williams is leading a study to understand the impact of slowing ovarian age on women’s health. The double-blind study looks at 100 women between 35 and 45 who have normal periods. (Double-blind means no one knows who receives the treatment and who receives the placebo.) Subjects are randomized to receive either rapamycin or a placebo. For three months, participants take a pill, and researchers will follow them for another nine months.
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Senben
#172
Hi! I started taking Rapamycin one month ago, 4mg weekly, as I am traying to delay menopause. I am 49 years old with more- less regular cycles. After taking Rapamycin for one month I measured my hormones and found out that my Estradiol levels are low. Could it be that Rapamycin suppressed my Estradiol levels as my body thinks that I am in deep autophagy (something similar to Calore Restriction so my body thinks there is no enough food and that’s the reason my body don’t produce enough Estradiol)? I read on this page about woman taking Rapamycin and having more regular cycles. Does it mean that more regular cycles could be achieved after taking Rapamycin for for example 3 months and than stopping taking it all together so that body doesn’t think it is in autophagy or one can get more regular cycles taking Rapamycin regularly (once per week)? Thank you
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Hi, and welcome to the website.
So - you did your Estradiol test After taking rapamycin for a month, but did you have any measures of your estradiol level before taking rapamycin?
I have not read deeply on the issue of impact on rapamycin on estradiol to know the exact impact. If someone else here has, please add your thoughts.
The research on rapamycin’s effect on fertility & menopause in humans is still in its early stages, so the short answer is we don’t know about the need for continued dosing. But in all the animal studies I’ve seen you generally see either continuous (daily) or pulsed (e.g. weekly) dosing ongoing over the lifespan if you want to keep seeing the benefits. There is some indication that people might be able to do dosing for a while, then take time off from dosing, and may still see the benefits. For example in some animal studies (on longevity, not on menopause or fertility) that I’ve seen, the dose for a few months, then they pause for a few months, etc. and the benefits continue for many months afterwards. But the first real study in humans as it relates to menopause has just been started at Columbia University Medical School and we won’t know the results for perhaps a few years. So its all still very early.
There are some good reports from people as can be seen in this other thread on the topic:
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Senben
#174
Hi! Thank you for your response…
I took my Estradiol test also before taking Rapamycin and results were in range of normal menstrual cycle - at day 7 of my previous period Estradiol was 45 pg/ml and on day 14 (before ovulation) it was 330 pg/ml. In this cycle (after taking Rapamycin for 1 month), Estradiol on day 7 is very low, it is only 20 pg/ml and its resembles menopause values.
Maybe I was takin too high dose of Rapamycin -(4 mg per week), and I weight only 52 kg.
Maybe Rapamycin pushed me in deep state oh autophagy and my body thinks there is no food so my reproductive system switch off and after some period Rapamycin will impact ovarian regeneration and my period will be normal with normal Estradiol values., The question is how long does it take for Rapamycin to regenerate ovaries and does Rapamycin disturbs menstrual cycle during regeneration (maybe in a firs few months)?
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Do you know when in your rapamycin cycle that you took the estradiol test?
For example, most of us here take rapamycin on a weekly schedule. If the day you took the estradiol test was the day after your rapamycin dose it might be very different than if you took the estradiol test 6 days after dosing rapamycin because rapamycin typically has a pretty long half-life of around 63 hours.
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Senben
#176
I didn t know that. Thank you for telling me.
Yes, I took Rapamycin approximately 36 hors before I measured Estradiol.
Maybe I should do another Estradiol test next week but I will not take Rapamycin approx 4 days before taking Estradiol test,
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Sirt6
#177
Dear fellow women,
I’ve been on Rapamycin for 2 years now with no adverse effects. For contraception, I’m using a copper IUD. It’s now time to replace the device, so I did some research on what’s new on the market.
Problem: A copper IUD activates immune cells within the uterus, which is part of how it works. Sperm and possibly fertilized eggs are partly hindered by this immune activation. Anyone on immune suppression or medication for rheumatoid diseases should not rely on copper IUDs.
In addition to the inflammatory response, copper ions from the copper IUD hinder sperm, prevent the build-up of the uterine lining, and inhibit the implantation of a fertilized egg.
I’m sure there isn’t any data on anti-aging doses of Rapamycin combined with copper IUDs. We certainly shouldn’t increase the dosage to a point where mTORC2 is suppressed.
Worst case: I don’t know if it’s possible to become pregnant if ovulation has just occurred, Rapamycin is taken nearly at the same time, and sperm enters the uterus. I also don’t know, if the lokal immune activation near the Cu IUP follows a curve when combined with rapamycin for anti aging.
Any thoughts?
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Senben
#178
Just wanted to report that I took another Estradiol test after skipping weekly Rapamycin dose and Estradiol was in normal range. It seems that Rapamycin just delayed my cycle a little bit.
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It seems that if rapamycin does in fact delay menopause it would help with reducing risk of dementia:
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The New York Times covering this area now: Is Delaying Menopause the Key to Longevity? (NY Times)
In the article, they state that in the Columbia University study they are tracking AMH hormone levels in women (available in most areas via an inexpensive blood test as described below) to track effectiveness of rapamycin as an intervention in this area.
For women taking rapamycin for improved fertility or menopause prevention it seems like we may be table to track if its working by monitoring AMH levels. Please consider taking the AMH test and please also report back your results. This way we could get some early indications of how well its working and not have to wait for the next few years while Columbia University completes its research and publishes the results.
A clinical trial currently running at Columbia University is also trying to slow the rate at which women lose their eggs. The study is testing the use of an immunosuppressive drug called rapamycin — which is used to prevent organ transplant rejection and has become a darling of the longevity movement — in women between the ages of 35 and 45 to see how it affects their ovarian reserve. Rapamycin influences the number of eggs that mature each month, and the drug has been shown in mice to extend ovarian function.
The study is still ongoing, and the researchers don’t know which participants received the medication or a placebo, but the lead scientist on the trial, Dr. S. Zev Williams, said that two patterns had already emerged: Some women appear to have a normal decline of ovarian reserve, which can be measured via ultrasounds and AMH levels, but in others, “it seems to have been altered,” he said. “So, you know, that’s promising.” Dr. Williams, an associate professor of women’s health at Columbia, is also applying for the health agency funding.
The experts were explicit that the goal of this type of research was not to prolong women’s periods indefinitely, nor to make pregnancy possible at age 70 — though the treatments could potentially extend fertility.
Read the full story: Is Delaying Menopause the Key to Longevity? (NY Times)
AMH Levels with Age:
it would be really interesting to know if (if you ever do this testing) how your AMH levels compare to the typical trendiness for females (see following graph). If we see significant outliers in the rapamycin group (higher than expected) it would be a very good sign that rapamycin is doing what we think it should be doing.
| AMH levels according to age for women 24-50 years of age. Median values are shown with standard deviations. Reproduced with permission from Seifer et al. Age-specific serum anti-Mullerian hormone values for 17,120 women presenting to fertility centers within the United States. Fertil Steril. 2011; 95 (2): 747-50.
Mean values of AMH of three different tests as a function of age. We can see the decrease in the mean values of each consecutive test placed on the market.
Source: https://www.researchgate.net/figure/Mean-values-of-AMH-of-three-different-tests-as-a-function-of-age-We-can-see-the-decrease_fig2_261746114
How to get an AMH Test
Just order it online (if its available in your area) then go to the LabCorp or Quest facilities to get the test done. Its quite inexpensive. (check to see if which lab the online service uses to fulfill the blood draw, make sure there is one in your area).
Marek: $65
UltaLabs: $87 at current listing
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Hello. According to the Vibrant study, to delay menopause, 5 mg should be taken every morning for 3 months.
Is it advisable to rest for how long after that period?
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Hi Victoria, welcome to the site.
We really don’t have good data on if a break should take place, or how long. One factor to consider is how your body is processing the rapamycin. You can take a rapamycin blood level test (on the last day before you take your weekly dose) and check to see what your blood-sirolimus level is. If you still have a meaningful (i.e. over 1ng/ml) level of sirolimus (rapamycin) in your blood then it may be that you are not fully clearing rapamycin from your system each week (in which case a break for a number of weeks may not be a bad idea).
I recommend you review these threads as part of your decision making process:
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