I’m into the DNA analysis topic for my protocol software development at hackingbiology, and i found out a lot of services that provides you acquisition and analysis of DNA for various purposes.
What i do want to ask to experts here is what would be the best way to split DNA data acquisition from DNA data analytics?
Specifically i understood that we could be interested into two different DNA analytics such as:
- DNA Genome Sequencing
- DNA Methylation pattersn
If you had to request a lab to bring you one or two files of your DNA (for sequencing and/or for methylation patterns), what do we need to request?
And step-2, most important, what piece of software or library or online services could we uses to come up with specific aging-related analysis (including hyperexpression or hypoexpression of specifics genes) ?
Thanks for the input, i’d love to really separate sequencing from analysis of data.
Fabio
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Hi Fabio,
I recommend you review these past discussions on related topics:
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Thank you, i’ve been reading previous posts, what i am not really (yet, being a new domain i just opened), is the kind of DNA analisys taking into accounts Epigenetics.
While WGS let you knows “who you are - what’s your gene”, my understanding (but i maybe wrong) is that to understand “what’s your current DNA evolution status” it’s needed to goes for WGBS (whole-genome bisulfite sequencing) that’s a very different analysis.
On this (getting deeper on epigenetics too) i have not yet come up with an understanding
Fabio
WGBS would bring exactly which genes (at CpG Sites) is methylated:
All with high level output according to sector data standard Whole-Genome Bisulfite Sequencing Data Standards and Processing Pipeline – ENCODE :
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