Agree. This looks like a honeypot to me. A great way for the powers that be to collect data on sources to shut down directly (US gov) or indirectly (trade pressure on China). I’m sure Eli Lilly and the FDA love this site.
“Our investors understand the importance of creating reliable supply chains for health and wellness products, and they’re committed to supporting our vision for a safer marketplace.”
Translation: provide us with free data that we can use to justify crackdowns. When those are over, we’ll gladly supply you with “safe” peptides and nootropics from our FDA approved (bought off) pharmacy at 20x Chinese prices.
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This is one of the things there seems to be a bit of confusion on. The real peptide mfg all indicate >95% to >98% purity. You can see this on actual research chemical websites. The 99% is marketing number. Basically anything over 95% is more than pure enough for human use.
Also the assay number is helpful. This is the number that tells you how much of the peptide itself is in the total amount of powder.
For those selling prepackaged peps, there can be up to 90% filler.typically mannitol with 10% active ingredient, the peptide. Once dissolved in diluent, those numbers don’t mean much as the dosing regime is the same, you just get lots of basically harmless “filler” with your injection.
For those selling the “raw” peptides there will be less than 10% of the powder that is not the 98% pure peptide. So what is the extra 10% in this case? typically harmless amino fragments.
People may think all that is in the vial is 99% pure peptide when that is not true in either case.
1 Like
qBx123Yk
#862
No disagreement here. In fact, the 99% guarantee only applies to GLP1s for most vendors. But if you test a GLP1, and it doesn’t fall under the vendor guarantee for purity, then tough for them: they’re the ones that made the promise to refund/credit, not you.
Yes, of course. Some people who don’t know better will often propose to weigh a vial of lyo powder and deduce the peptide weight based on that.
Yes, purity, refers to the amount of peptide that has not degraded (which means you have to know what peptide you’re looking for in the first place), not the amount of powder in the vial. Your peptide can come with other stuff like endotoxins, or the presence of heavy metals, etc.
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Tim
#863
What does PeptideTest charge per batch or vile? Janoshik is in the $200-300 range, which used to be reimbursed by the vendor in the form of credit.
Tim
#864
I volunteer.
The wide variance in quality may explain why one user had good results and another user did not.
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Davin8r
#865
$300 for the standard incretin mimetics, somewhat less for GH-releasing peptides.
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A bit more info on this peptide - also known as TB500 (frag 4) and Goralitide
https://www.ahajournals.org/doi/10.1161/hyp.70.suppl_1.p289
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Tim
#867
Good papers, Steve. They help explain why the benefits I get from TB-4 are not imaginary.
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qBx123Yk
#868
Tirzepatide and semaglutide are now on the WADA 2026 monitoring list. I suspect this list will get longer soon if they’re going that way.
1 Like
LukeMV
#869
More on TB500 from Vera AI. Note I was using it as part of a KLOW peptide blend ever since having shoulder surgery and decided to stay on indefinitely.
Thymosin beta‑4 (TB500) is a synthetic peptide fragment of the naturally occurring thymosin β4 protein, investigated for tissue repair, angiogenesis, and anti-inflammatory effects. Evidence is largely preclinical, with limited early-phase human data.
Benefits (Potential, Evidence Mostly Preclinical)
-
Cardiac repair and angiogenesis:
- In a porcine myocardial infarction model, TB4 enhanced engraftment of transplanted cardiomyocytes, improved left ventricular systolic function, and reduced infarct size without increasing arrhythmia or tumorigenesis 1(Theranostics, 2021; Level II evidence).
- In rats, systemic TB4 reduced infarct size and improved hemodynamic performance post-MI 2 (Front Pharmacol, 2013; Level II evidence).
-
Wound and tissue healing:
- Accelerated mucosal wound closure and angiogenesis in rat models 3 (Int J Mol Med, 2014; Level II evidence).
- Improved survival of random skin flaps and fat grafts by enhancing microcirculation and VEGF expression 4 (J Reconstr Microsurg, 2015; Level II evidence).
-
Neuroprotection:
- In traumatic brain injury models, TB4 reduced lesion volume, enhanced neurogenesis, and improved cognitive and motor recovery 5 (J Neurosurg, 2012; Level II evidence).
-
Pilot human data:
- EPCs pretreated with TB4, transplanted in acute STEMI patients, were feasible, safe, and suggested improvements in LV function and exercise capacity 6(Cytotherapy, 2016; Level II evidence, pilot trial).
Downsides / Limitations
-
Lack of robust clinical trials: No large RCTs confirm efficacy or safety in humans. Evidence is almost entirely animal-based or early pilot studies.
-
Unknown long-term safety: While no tumorigenesis was observed in large-animal MI models 1, theoretical concern exists due to TB4’s role in cell proliferation and angiogenesis, which could promote malignancy in predisposed tissues.
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Variable efficacy: In Duchenne muscular dystrophy mouse models, TB4 increased regenerating fibers but did not significantly improve overall muscle strength or cardiac function 7 (PLoS One, 2010; Level II evidence).
-
Regulatory status: TB500 is not FDA-approved; clinical-grade formulations are unavailable, and most products are unregulated research peptides with potential contamination risks.
Summary Table
| Potential Benefit |
Clinical Evidence |
Supporting Data |
| Cardiac repair post-MI |
Animal (porcine, rat); Pilot human EPC study |
Improved LV function, reduced infarct size 1 6
|
| Wound/tissue healing |
Animal (rat, rabbit) |
Faster closure, angiogenesis, improved graft survival 3
|
| Neuroprotection post-TBI |
Animal (rat) |
Reduced lesion volume, improved recovery 5
|
| Anti-fibrotic effects |
Animal (lung, liver, kidney models) |
Reduced fibrosis 8
|
| Downsides |
No RCTs; unregulated formulations; theoretical tumor risk due to angiogenesis |
Limited human data; safety unknown |
Conclusion:
TB500 shows promising regenerative and cardioprotective effects in preclinical studiesand limited early human pilot data. However, no high-quality RCTs exist, long-term safety is unestablished, and risks such as tumor promotion and unregulated product qualityremain major concerns. At present, its use should be considered experimental and restricted to controlled research settings.
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About 3 weeks ago I modified my daily (morning) healing stack from BPC 157 500mcg + TB500 (frag 1-4) 500mcg by adding 2.0mg of GHK-cu every morning
And now, after reading this I’m going down the mouse hole LoL!
I’m going to switch one peptide in my healing stack from TB500 (frag 1- 4) to Tβ4 full and see if the shiny “spot” on the top of my head gets smaller 
From www.perplexity.ai
Full-Length Tβ4 and Hair Growth
Full-length Thymosin Beta-4 (Tβ4) has been shown to promote hair growth and hair follicle (HF) development. Studies using transgenic mice that overexpress Tβ4 demonstrate faster hair regrowth and increased hair shaft numbers compared to wild-type mice, while knockout mice lacking Tβ4 show slower hair regrowth and fewer hair follicles. Tβ4 appears to stimulate the expression of vascular endothelial growth factor (VEGF), which then activates downstream signaling pathways including MAPK/P38, MAPK/ERK, and PI3K/AKT, processes associated with enhanced hair growth. This leads to a faster hair growth rate, alterations in HF patterning, and increased hair follicle proliferation.
Further, Tβ4 promotes hair follicle stem cell activation and early differentiation of epithelial progenitor cells, and upregulates extracellular matrix components such as laminin 5, essential for hair follicle integrity. Topical application of Tβ4 in animal models has resulted in faster, thicker hair growth notably in mice and rats, including improved hair regeneration in aged animals. Collectively, these findings indicate that full-length Tβ4 facilitates hair growth primarily by influencing follicle stem cell activity, growth factor signaling, and matrix remodeling.[1][2][3][4][5]
⁂
https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.16241
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Lance Hitchings on how to build a peptide stack.
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Tim
#872
No, you’re chasing the ghost flames that appear over marshes and bogs. I’ve been using both TB-4 and TB-full, the latter with BPC and GHK, for more than a year. There has been no hair growth, not even fuzz. Your higher doses may bring different results. And hey, there are other benefits, so give it a try.
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From what I’ve read it needs to be injected in the scalp or micro-needled.
I’ve ordered some of this vials with the micro needle caps to try this approach.
I’m hoping the ghost flames don’t take what’s left LoL!
I don’t expect much at all though, just curious.
I have been using Goralatide/TB500 (frag 1-4) continuously for a couple years with good results for healing but I also have Thymosin beta 4 (Tβ4) so I might as well put it to use. Will be ordering some TB500 (frag 17-23) in a couple weeks, again out of curiosity So I’ll have the 3 versions on hand.
2 Likes
Nick1
#874
Where is thymosin alpha 1 being sourced from? Anyone can direct message me if they would like to. Thx
What was your dosing protocol?
To follow the clinical trial results I need 20mg per day so I take 10mg twice a day, morning and dinner time. The boss started as well a couple days after me…
I added a “confounder” ARA 290 at 4mg per daily dose. But as the head guinea pig for the fam, it’s my lot to try anything before fam or clients
I don’t have any of the conditions listed, I got this one in for our 51y/o daughter who has suffered from an allergic reaction to Ciprofloxacin, one of the chronic symptoms is peripheral neuropathy.
ARA-290_benefits-point-list.pdf (75.1 KB)
Tim
#876
I took 5 mg, half a vial, every day for 20 days. Had a renal panel done and no change in GFR, which was my aim. SS-31 is said to increase GFR in a dose-dependent manner, with the best results coming from daily mega doses of 50 mg, which is cost prohibitive.
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You’re probably better off trying a low dose of retatrutide on terms of price and efficiency to see real egfr improvements.
Mine went from the mid 70s to low 100s in about 6 months. My dose varies from 4 to 6mg.
https://www.sciencedirect.com/science/article/pii/S2468024925001925
I think that dose was too low for the potential benefit. The cost is not inconsequential.
This is one of the criteria I use when I’m going to evaluate a compound, is the clinically demonstrated dose cost prohibitive? If it is I generally won’t do it. Follistatin 344 is like that. The dose is high, the benefit is low and it’s one of the most expensive peptides going.
On the other hand FOX04-DRI requires a high dose, the potential benefit is high and it’s the second most expensive peptide we carry, just behind FS-344
Something (ARA 290 or the SS 31) seems to have restored my ability to focus on deep work. Over the past 4 or 5 years I’ve found it more difficult to keep my head in a task for more than an hour, sometimes less. It was becoming a stress point for me.
I’ve done a lot of product dev over the years and used to be able to work 8 to 10 hours a day on detailed, structured work with minimal breaks. Day after day, weeks into months. One project took nearly 2 years of this type of work. Some people seem to be able to do this for decades, not sure I could have ever done that.
I’ve found in the past 2 weeks that I can do what I used to do, focus for 3, 5 and more hours on detailed work that I was beginning to avoid due to this loss of focus. And be able to leave projects that take multiple days to get through and get back to the task with more insight.
I’m a bit excited about where I am at this time.
3 Likes
Satchel
#879
My mental horsepower is way way way down. What dose of ARA/SS are you trying? and where in Canada are you finding these peptides? I don’t see them on BioTech Chem site?
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