#21

1ug/kg is way, way too much.

I got the dose guidance from Steve Perry who is the gdf11 expert and now also uses klotho, he initially found the dose with the help of ex cycle champion Marcello Bergamo.

skip down to the end of the document for dosing, here is the blurb.
Dosing Klotho
Given the side effects of Klotho, an extremely conservative approach to dosing is warranted.
I recommend you take it very slowly and start with .2 pg/day. Being a metabolic hormone, daily dosing is recommended. You certainly can’t take insulin or thyroid once or twice/week and the same holds true here. My current dose is .5 pg/day and almost everyone in the GDF11 cohort takes this dose.
Until you perfect your dose, do not take Klotho on an empty stomach or while fasting. Hypoglycemia will result which can be dangerous. If you experience this, drink fruit juice, or eat something ASAP.

4 Likes
#22

I found 3 labs that offer a Klotho blood test, generally quite expensive:

  1. Klothoyears offers a home test kit for $1059 (+ phlebotomist fee) : Klotho Test – Individual | Klotho Years (They also offer a “crowdsource” version of the test for $459 : Klotho Test – CrowdSource | Klotho Years)

  2. Jinfiniti offers it as part of their most advance panel, $1198 each, but 4 test panels are regularly $4192, and all tests were another 25% off on Memorial day or Black Friday, or 20% off with coupons at certain other times, I just paid $3144 for 4 test kits on Memorial Day: AgingSOS® – Advanced Panel - Jinfiniti Precision Medicine. In addition to Klotho this panel includes several other Aging tests that are hard to find or expensive on their own, including NAD, beta-Galactosidase (specific marker of senescent associated SASP), 8-OHdG (for DNA damage from ROS), Glycated Serum Protein (more accurate than A1C for measuring AGE glycation) and Interleukin panel (IL-1,IL-6,IL-8,TNF-alpha, less specific for SASP or inflammation). This tests allows blood collection in your home, but you need to fill a small test tube by finger prick(!), so I recommend paying $35 or so extra to have the blood drawn by a phlebotomist, unless you live in some state that doesn’t allow this without an order from your own doctor (like NY)!

  3. A cheaper stand-alone test for $199 : https://longevitylabsolutions.com/?product=soluble-klotho-blood-test (Not available for NY residents). This company also offers other Longevity panels (somewhat similar to Jinfiniti), but keeps the Klotho test separate. You draw a small tube of blood in your home with a device that automatically sucks capillary blood from your upper arm for 5 minutes : https://longevitylabsolutions.com/wp-content/uploads/2023/06/Tasso_DeviceInstructions.pdf

4 Likes
#23

While 1ug/kg may be way too much, how can 0.5pg/day make any difference given that the circulating levels of Klotho are around 2360 ng according to your link ?

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#24

I buy sterile bottles for injections. They have rubber seals. I buy sterile bacteriostatic water. That is water with an additive that prevents bacterial growth.
I inject 10ml sterile water into the klotho vial. Then I take 1ml out of that and inject it into the next vial. Then add 9 ml water. Then take out 1ml and inject into the 3rd vial and add 9ml water…and so on until I reach the desired concentration. It’s called “serial dilution”.

Eventually I get to a solution of 2ng/ml. I inject 0.2ml once per week.

So I’m using 400picograms/week. This is way more than Keith’s dose. I used to dose the same as Marcel and Steve but I noticed no effects at all. So I increased.
The last time I had a blood test was just over a year ago and my plasma phosphate level, which was averaging 1.09 mmol, had decreased to 0.58. The normal range is 0.8-1.5 mmol.
Serum phosphate tends to increase with age. Klotho tends to decrease with age. Klotho aids the excretion of phosphate.

I have posted all this stuff on other threads. I regard serum phosphate levels as an indicator of klotho status. I’m not going to post it again.

THIS IS NOT A SUGGESTION THAT ANYONE ELSE READING THIS SHOULD DO THE SAME!
Do your own research. Read the papers. Get blood tests done. What worked for me may not work for others.

The last thing I want is to be lectured to by some sanctimonious MD.

10 Likes
#25

There are (expensive) tests available for Klotho. I don’t use them. If you are my age (67) and otherwise normal trust me; you’ll be low on klotho.
Read my other post in this thread about how I use serum phosphate as a de facto indicator of klotho status.

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#26

Hi RapAdmin,

If you go to www.mimotopes.com and look at their FAQ you’ll see it.
There are heaps of other sources. Just type “what are the main impurities in synthetic peptides” into Google and you’ll find them. There are some slight variations but that’s mainly what the impurities are.
Cheers
DrT

3 Likes
#27

No idea, but that sounds like an irrelevant question as we are way past that needing to make sense as I am telling the dose that has been found effective by me and many others for years.

1 Like
#28

I don’t disbelieve you, I only just started reading about Klotho. What I see in my initial readings is that exercise, vitamin D, rapamycin, ACE inhibitors, and statins increase Klotho. If I’m already doing/taking those things, will I still have a sufficiently “low” Klotho level that injections to increase it are worthwhile?

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#29

Well the only way to definitively answer that is to get tested.
However, I can tell you that I have exercised heavily for decades, I take Vit D, rapamycin and ACE inhibitors (not statins though). Nothing made any difference to my serum phosphate levels until I started injecting klotho.

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#30

Holy shitballs, Batman!

Well, at least I found where the thrill seekers reside on rapamycin news.

I guess I need to listen to the podcast to see what the hubbub is all about. But I’m not injecting 90% pure anything (in case anyone was worried).

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#31

The podcast really is a great one. Everyone should take a listen IMO. A single “low dose” injection of Klotho in primates increased cognitive performance for at least 4 weeks! Higher dose had no effect but also no apparent side effects. Seems like this is going to end up being a huge breakthrough (although I’m still not ready to start self-injecting, either).

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#32

I have been meaning to research this Klotho thing ever since I found out I have the “detrimental” Klotho variant SNP rs9536314(GG) (only 2% of population have it).

https://blog.kittycooper.com/2014/05/klotho-a-gene-for-longevity-and-alzheimers-protection/

I was going to try out Calcitroil or Paricalcitol (VDRAs), as other papers suggested it increased Klotho expression, I just never got around to ordering it. Now, I plan on testing my levels first, then add VDRAs and test again, then based on that info, inject Klotho and retest.

I’ll be the lab rat here :slight_smile:

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#33

This article says Klotho levels are raised in mice and humans by reducing Senescent cell load with D+Q : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034457/, though human testing was limiting to patients of IPF, known to be linked to high levels of senescent cells, so unclear if humans with normal levels of senescent cells would benefit.

DAV therapy seems to act as a very strong senolytic, even at 50% of the recommended dose for 3 weeks (or less), so I plan to test my Klotho levels after my recent DAV therapy (which raised NAD levels by an impressive amount, also an indicator of cellular senescence) , see my Dec 2023 post in this thread My DAV* Therapy begins! *Doxycycline, Azithromycin and Vitamin C - #445 by curt504

4 Likes
#34

Is the only test for Klotho levels the one from Klothoyears?

#35

You using Bucky Labs? I see they are OOS

#36

No, there are at least 2 other labs, see my post above from 2 days ago. The least expensive is for $199, but excludes residents of NY : They are located in Texas so you should be good.

1 Like
#37

I’m really interested in your Klotho test results! Let us know what the values are.

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#38

As it happens I had a complete blood test pending and just received the results today.
FWIW, some results that may be of interest are:

  1. My phosphate level is still lower than normal, but only just. Lower boundary is 0.8 mmol and mine was 0.74, but still the GP picked up on it. (Very low serum phosphate can cause cardiac arrhythmia.)
  2. ha-CRP was 0.57 Mg/L. This has trended steadily down since a reading of 3.0 in 2016.
  3. Fasting insulin was 2 mU/L; same as last year.
  4. My lithium value was low <0.1 mmol/L. Reference range is 0.5-1.0. This came as a surprise as I had been taking 15 Mg Li-Asp per week.
  5. My Uric acid value was 0.27 mmol and has trended down each year since 2018. Reference range is 0.2-0.5. I need to go check if this is significant or not.

Comments or questions welcome.

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#39

I just checked; apparently lower serum Uric acid is “good” as it is a bio marker for longevity. So I’m naturally pleased about that.
The observations from my blood test results strengthen my belief that klotho supplementation maintains kidney function in old age.

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#40

Correcting Acidosis in CKD with a sodium bicarbonate supplements also restored normal levels of urinary alpha-Klotho levels over 4 weeks, though plasma Kotho levels did not improve significantly : https://www.sciencedirect.com/science/article/abs/pii/S1051227618302498

Presumably sodium citrate would also correct Acidosis in CKD.