#440

That is my experience also. Actually, I feel it’s made my immune system stronger. I don’t think medical community has understood all the mechanisms of RAPA. It may negatively affect certain bacterial infections, but I definitely don’t feel that my immune is weaker.

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#441

I would also agree that since starting Rapa in May I have been less prone to viruses and infections. I have just had two in rather short order! However, as I am asthmatic anything like this usually goes to my chest but thankfully neither of the viruses I picked up recently has been any more than a slight annoyance. Was this protection from the Rapa? Maybe. It’s hard to know given all the other stuff I have been trying over the last couple of years!

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#442

I’d second that. I believe that rapamycin has strengthened my immune system. I seem to have fewer colds and when I do catch one, it’s very mild. I even dodged Covid when the rest of the family caught it.

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#443

I started my DAV adventure today (12/31/23) to hopefully join the people who have completed the protocol: KFISH, Joseph, blsm, Rmun, Walter_Brown, Vjha, Goran, essexaid, J0hn, desertshores, Lowroad. Mostly they report no side effects but also no (obvious) changes. (I picked today to start so I could easily use @desertshores handy schedule which starts on a Sunday (#240 in this thread ). Hoping not to be one of those (couple of) people who had to stop because of stomach issues. Any hints to avoid such problems would be welcome! I have the meds, so why not give it a try?

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#444

I am surprised that anyone had stomach issues because I felt absolutely nothing from the DAV therapy.
I hope I obtained some hidden benefits.

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#445

DAV: any noticable benefits??

I read / scanned this thread. Appologies if I missed any comments from post DAV takers and their 1st hand seen benefits?

I’m sitting on a pretty good pile of Dox/Azi from a bulk buy from India of their Covid ivormectin protocol that includes Dox (or Azi) following the FLCCC(??) prophelatic / restorative protocol. Sooo Dox or Azi seems to have some systemic / immune up-regulation effect that the FLCCC folks knew about unrelated to anti-aging, senolytics or clearing CTCs.

But 100mg Dox/250 mg Azi (or vis versa) to me is not low dose. Its ok to ignore this ask if nothing to report. This thread is long enough.

Best wishes to all and thanks to the self-guinee pigs here as we all are in one form or another. Curt

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New Attia podcast on Klotho
#446

I just started a 50% DAV protocol (125mg Azi, 2x/week, 50mg Dox 2x/day and 1000mg LivOn Lipo VitC 2x/day) and will monitor results after 3 weeks and 5 weeks using this blood test for SASP : AgingSOS® – NAD, Senescence & Inflammation - Jinfiniti Precision Medicine

The test is not cheap (4 tests for $1992, currently running a 20% discount for New Years) but seems to be the only test available to measure Senescence levels (SASP inflammation). For those without cancer, lowering Senescence levels should be the primary effect of DAV.

I bought the 4 pack some time ago and used the first one a couple months back to see how well I was doing with Rapa (20mg every 2 weeks) and Renue Lipo Fisetin (1500mg/day for 3 days every month). My values on Oct 17, 2023:

Beta-Galactosidase : 853 (normal < 750) : This is the best marker for SASP inflammation
Interleukin 1-beta : 0.0 pg/mL (normal < 1.0)
Interleukin 6 : 4.4 pg/mL (normal < 3.0)
Interleukin 8 : 30 pg/mL (normal < 20)
Tumor necrosis factor alpha : 12.7 pg/mL (normal < 12.0)
Intracellar NAD : 65.1 uM (recommended 40-100)

(NOTE : NAD is not related to Senescence but included with the test and I supplement with 500mg Lipo NMN and 600mg Lipo NR per day, otherwise my baseline NAD was around 27)

I will follow up with values after 3 weeks (Jan 15) and 5 weeks (Jan 29). My thinking is that the effect on senescence should be noticeable after 3 weeks otherwise my 50% DAV is not working : If that is the case I will skip the test after 5 weeks and restart with the full DAV protocol after 6 months. If there is improvement after 3 weeks, it will be interesting to check if there is any further improvement after 5 weeks : If not, it may be possible to shorten DAV to 3 weeks, if targeting Senescence only.

I plan to use high dose pro-biotics (1000 billion CFU/day for 2 weeks) after completing my DAV, in case the gut biome was damaged using the following 11-strain product : 11 Strain Custom Probiotic Powder, then continue at 250 billion CFU/day of the 11-strain mix and add 250 billion CFU/day of their B.Lactis LL-4 (very similar to BB-12) plus 25 billion CFU/day of BB-12 from https://www.optibacprobiotics.com/product/bifidobacteria-fibre-probiotics

PS : I started the full DAV 1 month ago but stopped after 2 days when I got terrible abdominal cramping. I may have taken the Azi or Dox on an empty stomach and am avoiding that this time around. After 1 week, no problems at 50% of the normal dosage.

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#447

I’m glad I did it but regret not doing blood work before. My blood work after showed a pretty significant decrease in hsCRP even at 2 weeks after getting Covid fwiw. I continued rapa while on DAV. I plan on doing it again in October 2024. It felt really anti inflammatory to me and I sprouted some new hair.

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#448

Tried it twice but but had to stop by 4-5th day both times. Major GI issues and indigestion. I might be allergic to doxy perhaps.

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#449

Vitamin C and Doxycycline: A synthetic lethal combination therapy targeting metabolic flexibility in cancer stem cells
https://x.com/blagosklonny/status/1742549432338370795?s=46&t=ujBXvjsf5sfNM8J1qi8RfQ

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#451

That’s a helluva interesting article. Also of interest that MB is paying attention. It does lead me to wonder though, could this therapy be toxic to mitochondria in healthy cells as well? Or just put them under enough stress to induce mitophagy?

Also @desertshores I recommend you take a peak as it might get you to revise your opinion of Vit C as superfluous.

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#452

Thanks for the paper. I sent it to my daughter. She has a form of cancer that there is no good Chemo for. She’s been to the best. So has been treating with high dose vitamin C and a large stack of other stuff. When you guys started the DAV stuff I sent her the studies showing she needed to add Doxycycline. It took a while as usual, but she did it. The good news is that she is feeling well and had a recent surgery at Mayo and it may be gone. Gotta kill the CSC’s. She uses 75 grams vitamin C 3 times a week.

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#453

Google doxy+mitochondria on PubMed and there’s a lot along this vein:

https://scholar.google.com/scholar?start=10&q=doxycycline+mitochondria&hl=en&as_sdt=0,20#d=gs_qabs&t=1704332448952&u=%23p%3DUXuK5D3NLp8J

Tetracyclines, a class of antibiotics that target bacterial translation, are commonly used in research for inducible gene expression using Tet-ON/Tet-OFF systems. However, such tetracycline-inducible systems carry a risk. Given that mitochondria have a “bacterial” ancestry, these antibiotics also target mitochondrial translation and impair mitochondrial function. Indeed, treatment with doxycycline—a tetracycline derivative—disturbs mitochondrial proteostasis and metabolic activity, and induces widespread gene-expression changes. Together, this affects physiology in well-established model systems ranging from cultured cells to simple organisms and to mice and plants. These changes are observed with doxycycline doses that are widely used to regulate gene expression. In light of these findings, and bearing in mind the conserved role of mitochondria in metabolism and whole organism homeostasis, we caution against the use of tetracyclines in experimental approaches. The use of newly developed tetracycline-based systems that are more sensitive could be an alternative; however, even if no overt mitochondrial toxicity is detected, widespread changes in gene expression may sensitize cells to the intended tetracycline-controlled loss or gain of function, thereby introducing a “two-hit model.” This is highly relevant for cancer research, as mitochondrial metabolism holds a central position in the reallocation of nutrients for biomass production known as the Warburg effect.

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#454

Is she taking Vit C in an IV or oral form?

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#455

FWIW

The person an MD who did the most treating real patients was Dr. Hugh D. Riordan MD

Some of his work with protocols ( Riordan IV Vitamin C (IVC) Protocol) can be reviewed at

IV Vitamin C above 200g, Yes 200g per infusion

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#456

You can’t take very much orally, it doesn’t work. She does all IV.

#457

I think they’re talking about a system where they have genes that they can turn on with tetracycline. It’s a lab thing. We’re talking about a human with cancer.

They’ve used small amounts of doxy for years to stop acne. I think at these low rates, actually 50/day, this can force the cancer to use glucose for food, and when it’s using glucose for food vitamin C can kill it. If over time it drifts away to another food source, then the vitamin C won’t work.

I don’t actually understand this stuff very well and am not sure what you’re trying to say.

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#458

But I’m not sure there are no implications for clinical practice or consequences to healthy non cancerous cells. The way doxy gets cancerous cells to switch to glucolysis metabolism is by strongly stressing out their mitochondria so they no longer can use them. My question is what’s to stop it having this effect on the mitochondria of healthy cells too?

Read this new user’s consequences from antibiotics that similarly target mitochondria as a case in point:

Perhaps it has to do with dosage though. My concern is for implications of the DAV-C protocol though perhaps at the sub clinical dosages used those antibiotics only exert a mild hormetic stress.

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#459

My DAV adventure has come to an abrupt halt after 3 days! :frowning:

My heart started skipping beats on the 2nd and 3rd days, and by the end of the 3rd day was occurring quite frequently; I checked side effects for both azithro and doxy and irregular heart beat is a potential side effects for each. So after the 3rd full day I halted the protocol. The following day the skipped beat issue had largely (but not totally) abated, and now on 5th day virtually no skipped beats. Normally, I have to watch my hydration or I’m suseptible to skipped heartbeats.

Yes, I’m disappointed this didn’t work for me!

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#460

Thank you to reporting your side effects. It’s something that has to be taken into consideration.

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