#28

I don’t disbelieve you, I only just started reading about Klotho. What I see in my initial readings is that exercise, vitamin D, rapamycin, ACE inhibitors, and statins increase Klotho. If I’m already doing/taking those things, will I still have a sufficiently “low” Klotho level that injections to increase it are worthwhile?

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#29

Well the only way to definitively answer that is to get tested.
However, I can tell you that I have exercised heavily for decades, I take Vit D, rapamycin and ACE inhibitors (not statins though). Nothing made any difference to my serum phosphate levels until I started injecting klotho.

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#30

Holy shitballs, Batman!

Well, at least I found where the thrill seekers reside on rapamycin news.

I guess I need to listen to the podcast to see what the hubbub is all about. But I’m not injecting 90% pure anything (in case anyone was worried).

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#31

The podcast really is a great one. Everyone should take a listen IMO. A single “low dose” injection of Klotho in primates increased cognitive performance for at least 4 weeks! Higher dose had no effect but also no apparent side effects. Seems like this is going to end up being a huge breakthrough (although I’m still not ready to start self-injecting, either).

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#32

I have been meaning to research this Klotho thing ever since I found out I have the “detrimental” Klotho variant SNP rs9536314(GG) (only 2% of population have it).

https://blog.kittycooper.com/2014/05/klotho-a-gene-for-longevity-and-alzheimers-protection/

I was going to try out Calcitroil or Paricalcitol (VDRAs), as other papers suggested it increased Klotho expression, I just never got around to ordering it. Now, I plan on testing my levels first, then add VDRAs and test again, then based on that info, inject Klotho and retest.

I’ll be the lab rat here :slight_smile:

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#33

This article says Klotho levels are raised in mice and humans by reducing Senescent cell load with D+Q : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034457/, though human testing was limiting to patients of IPF, known to be linked to high levels of senescent cells, so unclear if humans with normal levels of senescent cells would benefit.

DAV therapy seems to act as a very strong senolytic, even at 50% of the recommended dose for 3 weeks (or less), so I plan to test my Klotho levels after my recent DAV therapy (which raised NAD levels by an impressive amount, also an indicator of cellular senescence) , see my Dec 2023 post in this thread My DAV* Therapy begins! *Doxycycline, Azithromycin and Vitamin C - #445 by curt504

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#34

Is the only test for Klotho levels the one from Klothoyears?

#35

You using Bucky Labs? I see they are OOS

#36

No, there are at least 2 other labs, see my post above from 2 days ago. The least expensive is for $199, but excludes residents of NY : They are located in Texas so you should be good.

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#37

I’m really interested in your Klotho test results! Let us know what the values are.

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#38

As it happens I had a complete blood test pending and just received the results today.
FWIW, some results that may be of interest are:

  1. My phosphate level is still lower than normal, but only just. Lower boundary is 0.8 mmol and mine was 0.74, but still the GP picked up on it. (Very low serum phosphate can cause cardiac arrhythmia.)
  2. ha-CRP was 0.57 Mg/L. This has trended steadily down since a reading of 3.0 in 2016.
  3. Fasting insulin was 2 mU/L; same as last year.
  4. My lithium value was low <0.1 mmol/L. Reference range is 0.5-1.0. This came as a surprise as I had been taking 15 Mg Li-Asp per week.
  5. My Uric acid value was 0.27 mmol and has trended down each year since 2018. Reference range is 0.2-0.5. I need to go check if this is significant or not.

Comments or questions welcome.

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#39

I just checked; apparently lower serum Uric acid is “good” as it is a bio marker for longevity. So I’m naturally pleased about that.
The observations from my blood test results strengthen my belief that klotho supplementation maintains kidney function in old age.

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#40

Correcting Acidosis in CKD with a sodium bicarbonate supplements also restored normal levels of urinary alpha-Klotho levels over 4 weeks, though plasma Kotho levels did not improve significantly : https://www.sciencedirect.com/science/article/abs/pii/S1051227618302498

Presumably sodium citrate would also correct Acidosis in CKD.

#41

“My lithium value was low <0.1 mmol/L. Reference range is 0.5-1.0. This came as a surprise as I had been taking 15 Mg Li-Asp per week.”.
Lithiumcarbonate is used for various diagnoses; dependent on renal function and other medication 3x300mg or 3x400mg daily are necessary to get lithium values of 0,8-1,2 mmol/l that are both safe and therapeutic for many people. (Li2CO3)

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#42

Too much lithium can be harmful to kidney function.
One must be very careful with supplementation. I’m sure there are bioavailability differences between LiCO3 and Li-Asp.

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#43

Is there any reason I can not or should not go overseas, maybe to Vitalia City, and do a small clinical trial of Klotho on Alzheimer’s patients?

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#44

I like that idea. Seems like it would be a worthwhile project. There needs to be contract research organizations (CROs) in Vitalia to do this type of thing across a broad number of potential therapeutic / longevity interventions. Everyone interested in doing this shouldn’t have to start from scratch getting clinical space and hiring the people experienced in doing clinical trials, even in a small/limited scope scenario.

Really, what we need is an SRI-like organization (smaller, and more focused, obviously) based in Vitalia that just focuses on longevity compound clinical trials / testing.

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#45

I got around to listening to the podcast this weekend. Fascinating!

We have a couple of users here - have they experienced any cognitive enhancement from taking klotho?

Those who have done extensive reading (especially @DrT), are you aware of any downsides or side effects? None were mentioned in the podcast that I can recall, just appeared that an “excessive” dose didn’t help.

I was concerned at the statement that a klotho level that was 30% lower than the mean level was associated with 30% mortality over five years. That is a compelling reason to get tested, and, if the results show you are low klotho, to begin injecting now without waiting for human studies.

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#46

Just off the top of my head: if klotho increases kidney clearance of phosphate, then one obvious danger is phosphate depletion. This can lead to cardiac arrhythmia which is very serious. As with all supplementation: MORE IS NOT NECESSARILY BETTER!
Be careful people!

4 Likes
#47

Listen further in to the podcast. it works differently in primates than in mice. In primates anything over a typical youthful dose did not work. The smaller doses worked at about 1/3 to 1/5 the amount needed to match a youthful level. Use too much and it doesn’t harm but doesn’t work. They also seemed to indicated that monthly injections would likely be what is needed, not daily.