#433

They said in study 20mg twice a day will work. Worth a try vs 100mg

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#434

Just a quick update on my DAV experiment which is due to finish tomorrow night. On day 2 I had a CBC/FBC (Complete / Full blood count). All was good except my Eosinophils were slightly elevated at 1500 (they should be under 500 and mine were way under this on my last FBC which was 6 months ago). At the time I assumed the elevation was due to having a virus so I then had the test repeated on day 14 of the protocol. I have only just had the results back and the Eosinophils were then sitting at 2500. The original virus was very mild and I was fully recovered within a few days. I am now wondering whether the raised Eosinophils are actually due to either the Doxycycline and / or the Azithromycin. I shall repeat the FBC in around 10 days. Iā€™m travelling at the moment (on a transatlantic cruise with no stops until Florida next Sunday!). And then Iā€™m heading down to Quito so will try getting a FBC done there.

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#435

I just finished my 5-week DAV treatment. Frankly not much to report. Fortunately all rather uneventful. Only some mild stomach issues during the first 7 days. I took daily pre & probiotics in an effort to mitigate any negative impact. Will probably repeat the DAV treatment in about 6 months.

Considering doing a D&Q treatment next month as I believe in the principe of senolytics.

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#436

I took a test approximately 2 weeks in and then another one when I finished the 5 weeks. It was not by design, my testing schedule just happened to work out that way.
I saw no negative effects on my blood work from the DAV protocol.
Your rise in absolute EOSINOPHILS is probably caused by something else.
The number you cite must be absolute eosinophils because most labs use percentages for eosinophils.
In any case, mine went down from 2.4% to 1.3%
Absolute eosinophils went down from 204 cells/uL to 107 cells/uL.

ā€œEosinophils, sometimes called eosinophils or, less commonly, acidophils, are a variety of white blood cells and one of the immune system components responsible for combating multicellular parasites and certain infections in vertebratesā€

I donā€™t pay too much attention to this one test as long as it is in the normal range because the measurement varies widely from test to test. The body is quite often fighting some infections like tooth decay, wound healing, insect bites, etc.

I would expect the DAV therapy to lower the eosinophils count.

Do any others completing the DAV therapy have any blood work results to share?

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#437

Iā€™m awaiting my lab results that were drawn 12/1/23. Unfortunately I became symptomatic for covid on 11/17/23 and tested positive on 11/20 basically right around the time I finished DAV so I had to delay my blood work. Itā€™s probably not going to be worth much but I really wanted to specifically see my lipid panel, HbA1c and insulin so I went ahead and got the all the labs done that I normally check. Iā€™m planning to do DAV again in October of ā€˜24 so Iā€™ll do a pre and post next time around.

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#438

Yes, my Eosinophil counts mentioned are absolute numbers rather than percentages. At the end of the DAV protocol I went down with another virus, probably as a result of taking Rapamycin while still on a cruise. Rookie mistake I suppose! Again it was quite mild but the snotty nose is still hanging around so I havenā€™t bothered getting another full blood count yet. Iā€™m also in the Galapagos Islands now for a month so Iā€™m not sure how easy it will be to get a blood test done anyway.

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#439

Hi All, my N=1, in my Bryan Johnson-esk kitchen sink protocol I take quite abit of quercintin, mushroom powders, colostrum powder, zinc 50mg, selenium, and curcumin (BPC-95), all are perported to be anti-most sicknesses and every 2 weeks higher dose of rapa.

On a plane trip from EU a coughing kid behind us caughed the whole trip, and my wife tested positive for covid a day later, 2 days later I got a 1.5 day sniffles. I did not test.

IMHO rapa has not caused me to be MORE disease prone. And the short recovery I donā€™t know if it may be helping. Given the kitchen sink I canā€™t trace back to what does whatā€¦

No doubt Rapa vs diseases is probably per the individual, diet, protocols, sleep, BMI, insulin resistance, so many factors.

BUT a possible tip; when traveling use 1% iodine spray nasally and orally often. IE Povidone spray and many more names are now on the market. I keep a sprayer in my pocket. FWIW we also follow the FLCC (??) prophelatic covid protocol and predose with ivermectin (etc). Which given my wife got sick (non-rapa taker) is not fool proof. Both are in the canā€™t hurt ones protection scenario IMO.

Best to all.

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#440

That is my experience also. Actually, I feel itā€™s made my immune system stronger. I donā€™t think medical community has understood all the mechanisms of RAPA. It may negatively affect certain bacterial infections, but I definitely donā€™t feel that my immune is weaker.

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#441

I would also agree that since starting Rapa in May I have been less prone to viruses and infections. I have just had two in rather short order! However, as I am asthmatic anything like this usually goes to my chest but thankfully neither of the viruses I picked up recently has been any more than a slight annoyance. Was this protection from the Rapa? Maybe. Itā€™s hard to know given all the other stuff I have been trying over the last couple of years!

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#442

Iā€™d second that. I believe that rapamycin has strengthened my immune system. I seem to have fewer colds and when I do catch one, itā€™s very mild. I even dodged Covid when the rest of the family caught it.

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#443

I started my DAV adventure today (12/31/23) to hopefully join the people who have completed the protocol: KFISH, Joseph, blsm, Rmun, Walter_Brown, Vjha, Goran, essexaid, J0hn, desertshores, Lowroad. Mostly they report no side effects but also no (obvious) changes. (I picked today to start so I could easily use @desertshores handy schedule which starts on a Sunday (#240 in this thread ). Hoping not to be one of those (couple of) people who had to stop because of stomach issues. Any hints to avoid such problems would be welcome! I have the meds, so why not give it a try?

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#444

I am surprised that anyone had stomach issues because I felt absolutely nothing from the DAV therapy.
I hope I obtained some hidden benefits.

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#445

DAV: any noticable benefits??

I read / scanned this thread. Appologies if I missed any comments from post DAV takers and their 1st hand seen benefits?

Iā€™m sitting on a pretty good pile of Dox/Azi from a bulk buy from India of their Covid ivormectin protocol that includes Dox (or Azi) following the FLCCC(??) prophelatic / restorative protocol. Sooo Dox or Azi seems to have some systemic / immune up-regulation effect that the FLCCC folks knew about unrelated to anti-aging, senolytics or clearing CTCs.

But 100mg Dox/250 mg Azi (or vis versa) to me is not low dose. Its ok to ignore this ask if nothing to report. This thread is long enough.

Best wishes to all and thanks to the self-guinee pigs here as we all are in one form or another. Curt

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New Attia podcast on Klotho
#446

I just started a 50% DAV protocol (125mg Azi, 2x/week, 50mg Dox 2x/day and 1000mg LivOn Lipo VitC 2x/day) and will monitor results after 3 weeks and 5 weeks using this blood test for SASP : AgingSOSĀ® ā€“ NAD, Senescence & Inflammation - Jinfiniti Precision Medicine

The test is not cheap (4 tests for $1992, currently running a 20% discount for New Years) but seems to be the only test available to measure Senescence levels (SASP inflammation). For those without cancer, lowering Senescence levels should be the primary effect of DAV.

I bought the 4 pack some time ago and used the first one a couple months back to see how well I was doing with Rapa (20mg every 2 weeks) and Renue Lipo Fisetin (1500mg/day for 3 days every month). My values on Oct 17, 2023:

Beta-Galactosidase : 853 (normal < 750) : This is the best marker for SASP inflammation
Interleukin 1-beta : 0.0 pg/mL (normal < 1.0)
Interleukin 6 : 4.4 pg/mL (normal < 3.0)
Interleukin 8 : 30 pg/mL (normal < 20)
Tumor necrosis factor alpha : 12.7 pg/mL (normal < 12.0)
Intracellar NAD : 65.1 uM (recommended 40-100)

(NOTE : NAD is not related to Senescence but included with the test and I supplement with 500mg Lipo NMN and 600mg Lipo NR per day, otherwise my baseline NAD was around 27)

I will follow up with values after 3 weeks (Jan 15) and 5 weeks (Jan 29). My thinking is that the effect on senescence should be noticeable after 3 weeks otherwise my 50% DAV is not working : If that is the case I will skip the test after 5 weeks and restart with the full DAV protocol after 6 months. If there is improvement after 3 weeks, it will be interesting to check if there is any further improvement after 5 weeks : If not, it may be possible to shorten DAV to 3 weeks, if targeting Senescence only.

I plan to use high dose pro-biotics (1000 billion CFU/day for 2 weeks) after completing my DAV, in case the gut biome was damaged using the following 11-strain product : 11 Strain Custom Probiotic Powder, then continue at 250 billion CFU/day of the 11-strain mix and add 250 billion CFU/day of their B.Lactis LL-4 (very similar to BB-12) plus 25 billion CFU/day of BB-12 from https://www.optibacprobiotics.com/product/bifidobacteria-fibre-probiotics

PS : I started the full DAV 1 month ago but stopped after 2 days when I got terrible abdominal cramping. I may have taken the Azi or Dox on an empty stomach and am avoiding that this time around. After 1 week, no problems at 50% of the normal dosage.

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#447

Iā€™m glad I did it but regret not doing blood work before. My blood work after showed a pretty significant decrease in hsCRP even at 2 weeks after getting Covid fwiw. I continued rapa while on DAV. I plan on doing it again in October 2024. It felt really anti inflammatory to me and I sprouted some new hair.

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#448

Tried it twice but but had to stop by 4-5th day both times. Major GI issues and indigestion. I might be allergic to doxy perhaps.

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#449

Vitamin C and Doxycycline: A synthetic lethal combination therapy targeting metabolic flexibility in cancer stem cells
https://x.com/blagosklonny/status/1742549432338370795?s=46&t=ujBXvjsf5sfNM8J1qi8RfQ

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#451

Thatā€™s a helluva interesting article. Also of interest that MB is paying attention. It does lead me to wonder though, could this therapy be toxic to mitochondria in healthy cells as well? Or just put them under enough stress to induce mitophagy?

Also @desertshores I recommend you take a peak as it might get you to revise your opinion of Vit C as superfluous.

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#452

Thanks for the paper. I sent it to my daughter. She has a form of cancer that there is no good Chemo for. Sheā€™s been to the best. So has been treating with high dose vitamin C and a large stack of other stuff. When you guys started the DAV stuff I sent her the studies showing she needed to add Doxycycline. It took a while as usual, but she did it. The good news is that she is feeling well and had a recent surgery at Mayo and it may be gone. Gotta kill the CSCā€™s. She uses 75 grams vitamin C 3 times a week.

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#453

Google doxy+mitochondria on PubMed and thereā€™s a lot along this vein:

https://scholar.google.com/scholar?start=10&q=doxycycline+mitochondria&hl=en&as_sdt=0,20#d=gs_qabs&t=1704332448952&u=%23p%3DUXuK5D3NLp8J

Tetracyclines, a class of antibiotics that target bacterial translation, are commonly used in research for inducible gene expression using Tet-ON/Tet-OFF systems. However, such tetracycline-inducible systems carry a risk. Given that mitochondria have a ā€œbacterialā€ ancestry, these antibiotics also target mitochondrial translation and impair mitochondrial function. Indeed, treatment with doxycyclineā€”a tetracycline derivativeā€”disturbs mitochondrial proteostasis and metabolic activity, and induces widespread gene-expression changes. Together, this affects physiology in well-established model systems ranging from cultured cells to simple organisms and to mice and plants. These changes are observed with doxycycline doses that are widely used to regulate gene expression. In light of these findings, and bearing in mind the conserved role of mitochondria in metabolism and whole organism homeostasis, we caution against the use of tetracyclines in experimental approaches. The use of newly developed tetracycline-based systems that are more sensitive could be an alternative; however, even if no overt mitochondrial toxicity is detected, widespread changes in gene expression may sensitize cells to the intended tetracycline-controlled loss or gain of function, thereby introducing a ā€œtwo-hit model.ā€ This is highly relevant for cancer research, as mitochondrial metabolism holds a central position in the reallocation of nutrients for biomass production known as the Warburg effect.

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