#187

It’s probably the exercise for me. I track everything in cronometer to make sure I don’t go under but I’ve been trying to build muscle so I might need to increase my calories a bit. It’s definitely something I need to fine tune at this point.
image

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#188

Do you track calories too?
It is harder to gain weight when exercising I noticed. I wanted to put on some 3kg of weight, muscle weight to be exact. I train, I eat at enormous caloric surplus and after a month i am not sure I gained a single kg. I know that that defies the laws of physics but I eat at 4500 kcal surplus after two years of caloric restriction and here we are…

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#189

The other explanation is a worm infection in your gut, for example.

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#190

Worm infestation is more frequent than ppl may think, especially if one travels a lot or eats street food. I do not travel outside the US now. But to exclude a possibility, I periodically check myself for that because I live with a small dog. Even though she’s preventively on mebendazole, it’s a risk factor. All my tests show negative. The last was in March. So, it’s not a reason for me. I need to increase my food intake. Working on it. It’s not easy for somebody who is not used to eating much or after 6 pm. And I need only 5 lb to gain to feel good again :blush:

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#191

Single dose Rapamycin induces weight loss.

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#192

Yes but only to ensure I’m not in a deficit. I also feel good and I’m happy with my body composition fwiw. I think you’re right that my activity/exercise probably requires more fuel than I have been getting. The weighted vest I wear might be increasing my calories requirements more than I realized.

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#193

Just wondering, how can any of us determine whether this compound actually works to reduce cancer stem cells or other benefits? I saw someone mentioning blood work. Would that tell us and if so, what specific tests?

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#194

There are some tests that show if there’s cancer present.

There’s also Galleri test available (no insurance coverage) or full body MRI.

However there are no tests available to determine cancer stem cells at their initial stage before cancer starts. Or are there?

My understanding is that those who do DAV therapy assume that everybody has cancer cells that DAV therapy will take care of. Wondering how long the effect of elimination lasts? 2 mo, 6 mo, a year? How often one has to do that therapy to be cancer free all the time? How to compare before and after results? What if there’s a rebound mechanism with cancer cells (like with Rapa)?

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#195

This is my issue with this DAV therapy… there seems to be no obvious way to measure whether its doing anything positive. With rapamycin at least we have dozens of studies in all types of organisms, and mammals, that show lifespan improvement. With DAV it seems there is little data, and no way to measure help or harm. (though it does seem the risk is quite low, so I understand why people would want to try it).

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#196

RapAdmin:

Right, there is little data on DAV,. But hidden in amongst all the lab results from the doxycycline-only article was a small open window trial on doxycycline for just 14 days 200 mg per day on nine breast cancer patients. It showed reduced levels of two cancer stemness markers for eight of the patients.

It is fragile proof, since there seems to be no follow-up on the patients nor any further clinical trials. I tried repeatedly to get in touch with the author about this to no avail.

I will still go for doxycycline, only. Haven’t made up my mind whether to go for the 14 days of that study or the five weeks of the DAV protocol. Plus loads of pre- and probiotics.

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#197

Do you remember what the markers were?

#198

Review the paper(linked below) in detail, for the type of testing that was used.

It in NOT any “standard” testing.

Metabolic flux in real time.

You can duplicate the testing in the paper, it is not that difficult.

I did post about the equipment/analyzer.

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#199

Which paper, which author?

#200

Finally, my meds will be arriving shortly. I will be another canary in the coal mine.
I am having a wide range of blood work done this week to establish a baseline, but I don’t expect the DAV therapy to have any effect on my markers.

I find no compelling evidence that azithromycin is unsafe to take for a few weeks with the doses used in the DAV protocol.

“For 5 weeks, azithromycin was administered at 250 mg twice weekly, doxycycline at 100 mg twice daily, and vitamin C at 500 mg once daily. A 77-year-old man of interest had hair growth, mental cognition and agility, strength and stamina, and increased libido, as well as visual acuity, hearing, conversation, coordination and balance, overall well-being and vitality.”
Can’t pass that up!
“strength and stamina” certainly fits in with my current quest to gain more muscle mass.

I certainly would not recommend this to any of the younger users. But, I am in the Joseph camp. I am too old to wait for endless testing which may not be forthcoming for a while. It is the same with rapamycin, I am not waiting for extensive human trials.

In the meantime, I do not see anything alarming in taking the combo.

“Azithromycin: It is a macrolide-type antibiotic. It works by stopping the growth of bacteria”
They use it to treat infants and toddlers.
“The present analysis confirms that azithromycin can be safely used to treat bacterial infections in children of all ages.”

“500 mg azithromycin or placebo three times a week for 12 months”

“Azithromycin: It is a macrolide-type antibiotic. It works by stopping the growth of bacteria”

“3049 participants on azithromycin”
“We found no evidence that long-term use up to 2 years of macrolides or tetracyclines was associated with increased risk of SAEs.”

https://www.sciencedirect.com/science/article/abs/pii/S2213260014700190
https://accp1.onlinelibrary.wiley.com/doi/epdf/10.1002/jcph.2358

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#201

desertshores:

What about that study showing azithromycin use associated with significantly higher relapse of cancer, posted earlier by AnUser in this thread, is that not a cause of concern? (Maybe only relevant for those with cancer in remission like me…?)
.

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#202

The cancer stemness markers were CD44 and ALDH1. There are more markers but apparently only those two were tested.

#203

Michael Lisanti

Doxycycline, an Inhibitor of Mitochondrial Biogenesis, Effectively Reduces Cancer Stem Cells (CSCs) in Early Breast Cancer Patients: A Clinical Pilot Study

I called ML repeatedly, and left a message in the voice recorder plus in an email asking if there had been any follow-up on those nine breast cancer patients and whether some more clinical study was happening. No response so I gather there is none which may only mean he got no funding for it.

In any case, I am grateful to you for starting this thread since it made me go for doxycycline, even though I see it as a long-shot chance, also to help my own cancer stay in remission.

Am still pondering azithromycin, although I wish there were some human clinical data plus concerned with that cancer relapse result, even though it was after long use. Looking forward to hear about your results, when will we know?

#204

FWIW

“This combination therapy was designed to stimulate mitochondrial biogenesis, while simultaneously inhibiting mitochondrial protein translation, resulting in functional ATP depletion. This occurs because inhibition of mitochondrial protein translation effectively blocks the production of proteins encoded by mitochondrial DNA (mt-DNA), which are absolutely required for OXPHOS, thereby creating a “rho-zero-like” phenotype. Since Azithromycin is an established inducer of autophagy, this strategy should also stimulate mitophagy, to actively eliminate defective mitochondria. This functional property of Azithromycin may also have implications for aging (see Discussion).”

“Taken together, these results indicate that the combination of low-dose Doxycycline with Azithromycin might be a more efficacious therapeutic option than Doxycycline alone for the eradication of CSCs, by inhibition of mitochondrial function and glycolysis.”

Aging: Improving health-span and life-span

“We believe that the DAV triple combination therapy that we describe here may also have implications for improving health-span and life-span, as aging is one of the most significant risk factors for the development of many human cancer types [19,20]. Moreover, we have previously demonstrated that Azithromycin, by itself, is an FDA-approved drug, with remarkable senolytic activity, that targets and removes senescent fibroblasts, such as myo-fibrobasts, with great efficiency approaching nearly 97% [21]. The accumulation of pro-inflammatory senescent cells is thought to be the primary cause of many aging-associated diseases, such as heart disease, diabetes, dementia and cancer, to name only a few [21]. Since cancer-associated fibroblasts (CAFs) are senescent myo-fibroblasts, with tumor promoting activity, this triple combination approach with Azithromycin may also effectively target the glycolytic tumor stroma of aggressive and metastatic cancers, especially those bearing the metabolic hallmarks of the “Reverse Warburg Effect” [22–28].”

Above quotes are from;

Their are 32 REFERENCES in the paper you could review for more details.

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#205

@desertshores mentioned having bloodwork done beforehand to establish a baseline. What tests do people think are particularly worth getting before and after?

#206

No.
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