I would buy more GFJ and test a higher dose of GFJ and test again at 24 hours to see if you get a higher concentration that time. There were some studies that showed that some people need more than 200 ml of GFJ to get a decent increase in drug concentrations. So maybe double the amount of GFJ and see what happens.
3 Likes
adriank
#153
This week 12mg with 250ml. In two weeks 12mg with 500ml. God I hate GFJ!
Update:
Just had 12mg with 250ml GFJ. Blood test soon. Hopefully I’ll get over 20ug/L. I’m doing the test at 90 min.
Update 2:
Just had my blood test. I feel a lot more tired and colder than usual. I’m wondering if it is the nurse or the change in dosage or something else. I’m feeling hungry as I’m starting my fast. I had some watermelon this morning before the GFC. Don’t think those could have caused this weak feeling.
Update 3:
Since I managed to source fresh grapefruit, should I go with 2 or 3 average size grapefruit? thx. I will take it tomorrow and have 20mg and see what kind of results I will get.
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I can confirm that biocon rapacan contains active ingredients and is absorbed. Attached is my Rapamycin levels 2 hours after 6mg with food.
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This group - email Liz at help@hippevoshop.com offer, by request the LabCorp Sirolimus Level for $48.
Does anyone have experience with them? This is the lowest price I’ve seen. It usually costs me $105 through Quest.
Below is the info I got back from them enquiring on this. It looks to be self ordered also, so no physician requisition needed.
————————-
Our current price for LabCorp test 716712 Sirolimus – Whole Blood is $48.00
Below is the information that would be needed to purchase and create a lab requisition :
Patient name
Date of Birth
Phone
Address
电子邮件
Payment Information
*If they would like results sent to a Doctor, we would need Fax Number for Doctor
Thanks!
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Thanks basically that confirms that my siroboon works similar with the enteric capsules.
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Just got my LabCorp test back. Blood drawn 1 hr 45 min after taking, with a tablespoon of Udo’s Oil. 6mg Rapacan from an Indian pharmacy. Result was 16, so it looks like it’s the real deal. I take that once a week, have done so for probably 3 years.
4 Likes
Sirolimus Level Measurement and Dosing Strategy
I recently measured my Sirolimus level at UCK Gdansk, yielding a result of 2.4 ng/ml. This measurement was taken exactly 6 days after ingesting 3 mg of Rapacan (sourced from India) under the following conditions:
- Consumed one grapefruit 3 hours before dosing
- Took a shot of fish oil with the dose
- Followed up with a shot of olive oil 45 minutes later
The detection range for this test is 2-30 ng/ml. Given that “levels should drop below 1 ng/ml within 24-48 hours before the next dose” and considering the half-life of Sirolimus (62-80+ hours), I’ve calculated my optimal dosing interval:
- ~80 hours (3.3 days) for levels to drop to ~1 ng/ml
- Additional 1-2 days before the next dose
Total interval: 11 days between doses (6 + 3 + 2)I plan to maintain this dosing strategy. Any feedback or suggestions would be appreciated.
Previous Peak Level Test
In a prior test, I measured my peak Sirolimus level after taking 2 mg Rapacan:
- Result: 9.3 μg/l (equivalent to ng/ml)
- Timing: One hour after intake
- Conditions: Without grapefruit
Proposed Future Testing
Given that the upper detection limit is 30 ng/ml, taking 3 mg Rapacan with grapefruit would likely exceed this limit. Instead, I’m considering the following test:
- Dose: 1 or 2 mg Rapacan
- Conditions: With grapefruit and oils as previous test
- Timing: One hour after intake as previous test to compare
This approach could help estimate the multiplication factor of grapefruit on Sirolimus levels. My hypothesis is that it could be >3 and if so I should be taking only 1 mg. Would you recommend this testing approach or suggest alternative methods?
2 Likes
adriank
#159
Upper detection of 30? That is low. The labs here in Australia can detect much higher than that. Anyway, personally I think you need a higher dosage if you need to fix something. For maintenance, I take 10mg fortnightly with GFJ in line with a 85 hour blood test of over 4ng/mL. However, I feel that 20mg fortnightly with GFJ is better … but it is a huge spike in the first 2 hours… I took that dose weekly for nearly 6 weeks… and problems seems to clear up very fast. I am going to take 2x 10mg dose over the next 2x fortnights before ramping it up again.
jnorm
#160
I just realized Marek offered this test. It’s not that expensive either.
Curiosity is probably now going to get the best of me when it comes to testing the bioavailability of novel rapamycin RoA’s (IM, IR, IN). Does anyone have information regarding a non-toxic and effective solvent for IM injection? I’m thinking propylene glycol mixed with water might work.
See this thread: Intramuscular (IM) + Intranasal (IN) Rapamycin - A new paradigm for human longevity translation
I’m going to be doing a series of personal dosing studies, where I do the Marek blood sirolimus test at the 2 to 3 hour time period, then the 24 hour, then 48, 72, etc hour time periods to get a feel for my personal rapamycin AUC and curve. I’m also doing at the same time the Healthspan Starter Longevity Blood panel to track the impact (if any) on my key blood measures: Longevity Starter Panel
I’m going to start at 10mg/week and go up to 20mg per week and see what the effect is. Will report back as I complete it.
2 Likes
jnorm
#162
Are you going to take the Healthspan panel at each of these time points also? That’s quite a bit of testing, but the information would be very interesting. I think I’ll follow your lead on that.
Do you know any Telehealth providers offering the below test? Would provide information on how rapa effects the adaptive immune system.
Yes - perhaps not all, but all of the tests initially, then if numbers are not moving much I’d relax the testing schedule. Its only $40 for that healthspan test, so not too bad.
1 Like
I’ve been hunting around for that TREGs test (CD8, CD3, CD4, etc.)… and it seems that the Quest version is more available - see below. These are found by using the “find test” search engine in the previous post.
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Nick1
#166
Very helpful and intersting insights here with Rapa dosing, cycling and GFJ hac to inhibit CyP450.
While Attia and Matt K are often cited here.
Can anyone comment on:
- Is anyone following Bryan Johnson Protocol of Rapa cycling?
- He is leaning with but higher doses but I cant find what data is Bryan basing his dos9ng regimen on?
- Timing of GFJ. Maximum inhibition of CyP450 occurs 6 hrs following its ingestion. How is everyone timing their Rapa dosing as it relates to GFJ ingestion to get 3x multiplier effect.
Cheers!
Nick
1 Like
Agetron
#167
To be honest Nick… several on here like me literally take the rapamycin with the grapefruit juice simultaneously… I squeeze one fresh red grapefruit and get about 5 fluid ounces.
I get a 3 times increase 2.5 to 3 hours later with a LabCorp test.
I will run this protocol again December 4th for a trough first and C-max after dose…GFJ and 2.5 hour wait. Will share results here.
Most people here figure out their own dosing protocol based on their body’s response to rapamycin (any side effects at any given level?) - after starting low (e.g. 1mg/week) and working up from there.
Some people here have mentioned following Bryan Johnson’s protocol for a while, but Bryan recently announced he’s stopping rapamycin, see the thread here: Bryan Johnson stops rapamycin
Bryan doesn’t provide much detail on the test results / rationale behind his actions generally, but I think he was trying to balance out the higher dose (in theory more benefit at least at the levels we are typically dosing), and the long half-life of rapamycin.
On the timing of GFJ and rapamycin, I think most people take the GFJ a few hours prior to dosing and seem to get a good response, based on sirolimus blood level tests. You can also do this to test your results.
1 Like
It’s not 6 hours after ingestion. The maximum inhibition occurs from 0-4 hours after ingestion. That’s why it works well to take the rapamycin with or shortly after drinking the GFJ. As for the multiplier effect. It’s not 3x, it’s anything fro 0-8x. The average is just around 3.5x that’s why people refer to that. But in reality it can be far from that. That’s a major reason blood tests are necessary.
