How do I test levels of growth hormone signalling? (rapamycin does not interact favorably with GH KO mice)

[AlexKChen]

https://www.pnas.org/doi/10.1073/pnas.1717065115

There are fewer panels for it than even for IGF1

FGF21 inhibits it - Frontiers | FGF21 as Modulator of Metabolism in Health and Disease | Physiology

[AlexKChen]

Evidence that mTORC2 inhibition is detrimental, by Dudley Lamming

Rapamycin, when taking at high doses on a continual basis, is known to eventually start to inhibit mTORC2. One of the results of ongoing mTORC2 inhibition is immune system suppression (while this is considered a feature, not a bug, in organ transplant patients, in anti-aging its definitely a “bug” (negative). Research also suggests that mTORC2 inhibition shortens healthspan and lifespan in animals. These are the reasons why, for rapamycin in anti-aging, we typically take rapamycin on a pulsed dosing schedule (e.g. once per week or once every two weeks). Here is a twitter thread where one of the leading researchers on rapamycin and mTOR outlines the evidence on mTORC2 inhibition:

Walk in labs has a test…

Growth Hormone Blood Test Panel | Walk-In Lab

[AlexKChen]

I have 0.2 which is lower than most

Rapamycin is bad for GH KO though this is far from GH KO