Something that has system wide effects on a wide variety of cells and process is probably not inconsequential. Regardless of what lifespan results in mice says…
Targeting senescence or delaying aging is emerging as a critical health strategy for solving age-related diseases, especially in the old population. In the current review, we will delineate the mechanisms of organ crosstalk in systemic aging and age-related diseases to provide therapeutic targets for delaying aging.
https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202402479RRR
1 Like
Based on the following paper, an SGLT2I should be a part of senolytic therapies.
4 Likes
Tim
#286
I’m using FOX04-DRI, highly diluted because it has a sting. Taking it with MOTS-c, as advised by Alek Kikel.
I pulled a muscle today, but I expect peptide therapy to minimize the damage. I’ve recently added epitalon spray, which does promote extended sleep, to my repertoire.
I didn’t find that for me, and a friend who added Epitalon found the opposite effect.
I think the majority of people using this one do find the sleep benefit.
What dose are you using for Epit?
And what dose and schedule are you using for FOX04-DRI if you don’t mind me asking 
I’m going to up my dose for our next cycle April 1.
April is when we will also do our next Trudiagnostic test. Too many people getting ahead of us on the Rejuvenation Olympics leader board LoL! Over 5700 participants there. Joan is just barely in top 10 and at 50 and I’m out of the top 200 at 206. Still pretty good out of 5700 I think I may hook up with a Longevity Doc
1 Like
Tim
#288
The spray provides 10 iu per nostril, which is in the microgram range. Whether it increases telomerse length or not, it is said to retard the aging process. Maybe we’re all regressing, and that’s why we sleep like a baby. Or maybe, as somebody else once said, we have one thought less each year.
I take the FOX04 on an EOD schedule. I started with an 18-g vial, which I first diluted with 3 ml of bac water. It wasn’t enough to mitigate the sharp, persistent pain. I gradually upped the diluent to 9 ml, which gives me 2 mg/ml, more or less.
I know you suggest a saline solution for your spray. Any reason you couldn’t use bac water?
1 Like
No reason at all. From what I know about nasal sprays you could also use distilled water, I get that from Walmart, it’s dirt cheap I use that in our skin care products so I don’t need to use a chelator.
I do prefer a bit of “bacteriostatic” property in a nasal spray though.
That may reduce the risk of contamination, possibly? I also wipe the nozzle before and after use. Ya never know what’s lurking up there and I don’t want it growing in my spray bottle of a month LoL!
The one big BUT with nasal sprays is test the pH. I’ve had a bad experience when I made our NAC+Bromelain nasal spray.
2 Likes
Bac water has alcohol in it so I imagine it would irritate your nose cavity VS the saline
2 Likes
That is a good point. In my Selank and Oxytocin spray use Saline 0.9% Bacteriostatic 10ml Vial- With Preservative and dilute it with 10ml distilled water and no issues. I have not tried to use it full strength.
I use a BAC version as 1 full spray bottle will last about 1 month for 2 people and I’m just a bit concerned about stability.
2 Likes
Tim
#292
Yes, a little, but not bad.
Somewhere in this thread there is a study on old mice that when treated with senolytics had a positive effect on their testosterone output.
I started FOX04-DRI the end of Dec 26, 2024 and did one 6 day cycle of 3mg per day.
I’ve had 2 recent testosterone tests Dec 6 2024 and Mar 21, 2025 and one back in May 31, 2021.
had a good increase before starting FOX04-DRI and another little bump up after.
No TRT, just peptides. The only confounding factor would be that I also started Gonadorelin early in December.
I would say that made a difference and that quite possibly the FOX04-DRI is helping as well to enable the “boys” to function better with the addition of Gonadorelin.
We will be doing another cycle of FOX04-DRI next week and upping the dose from 3mg per day x 6 days to 4mg per day x 6 days.
Overall I’m pretty happy with these results considering it’s my boys doing the work. I will be getting another Test - test done in 3 months
4 Likes
