#21

But what if a large chunk of the research on SPMs is based on flawed science?

Last month, in a paper in Frontiers in Pharmacology, an international group of 18 scientists including specialists in lipid analysis argued that the studies identifying these molecules in people typically detect them at levels so low they are unlikely to play a major role in resolving inflammation. The critics also found that most studies of human samples merely correlate SPM levels with a phase during the arclike time course of inflammation; data proving SPMs actually resolve it are weak to nonexistent, the authors argue.

The paper’s authors offered an even more damning critique: Protocols developed by Serhan and his collaborators to detect SPMs in body fluids or tissue samples don’t conform to accepted standards, they say. What is provided as proof of SPMs in many papers should actually be interpreted as mere “noise,” they argue. “You cannot ignore the fact that there is a growing number of analytical chemists who cannot detect these things,” says Nils Helge Schebb, chair of food chemistry at the University of Wuppertal and lead author.

researcher Derek Gilroy of University College London, who has collaborated with Serhan but is now moving his lab away from SPMs, tells Science he has had a “very negative experience working with these things and seen some data that makes me question whether these things are real.”

The paper referenced:

Formation, Signaling and Occurrence of Specialized Pro-Resolving Lipid Mediators—What is the Evidence so far?

This paper is aimed at critically evaluating i) the proposed biosynthetic pathways of SPM formation, ii) the current knowledge on SPM receptors and their signaling cascades and iii) the analytical methods used to quantify these pro-resolving mediators in the context of their instability and their low concentrations. Based on current literature it can be concluded that i) there is at most, a low biosynthetic capacity for SPMs in human leukocytes. ii) The identity and the signaling of the proposed G-protein-coupled SPM receptors have not been supported by studies in knock-out mice and remain to be validated. iii) In humans, SPM levels were neither related to dietary supplementation with their ω-3 polyunsaturated fatty acid precursors nor were they formed during the resolution phase of an evoked inflammatory response. iv) The reported low SPM levels cannot be reliably quantified by means of the most commonly reported methodology. Overall, these questions regarding formation, signaling and occurrence of SPMs challenge their role as endogenous mediators of the resolution of inflammation.

5 Likes
#22

Thanks, @约瑟夫_拉维尔 and @RapAdmin, for the opposing views. May be worth trying anyway, but now my expectations will be down-shifted.

1 Like
#23

Thanks, @cl-user, I think I’ll order some.

Amazon has Thorne, which is $60 for 60 servings, and a few other brands. The least expensive is Life Extension, which charges $18 for 30 servings. A serving of both brands provides 300 mcg of pro-resolving mediators. I ordered the Life Extension, so I’m out only 20 bucks if it doesn’t work.

2 Likes
#24

I don’t have a dog in this hunt. I was looking for the original paper when I found the “maybe” not paper, which I shared here.

Good luck to all. Be sure to report back on results.

#25

Interesting indeed. Did you come across any risks or side effect that people mention?

Might be good to have a strategy around how quickly and in what context to step down supplemtation if you are in situation when you actually need your body’s inflammation response btw.

1 Like
#26

@Neo, Yes, I hope to find out whether these Amazon supplements have any resolvins at all, and whether these resolvins can actually resolve a case of aggressive inflammation, without completely suppressing the immune response. This seems like a question that will remain unanswered for years.

2 Likes
#27

In the Berry Sears video, posted by JuanDaw

Sears, states that using aspirin with fish oil would enable one to use larger doses of fish oil and it could then have even larger synergies in reducing inflammation.

Does anyone have any thoughts on optimizing the doses of fish oil vs aspirin?

1 Like
#28

Resolvin-D3-and-Aspirin-Triggered-Resolvin-D3-Are- copy.pdf (1.9 MB)

This paper speaks to the synergistic effect of aspirin and omega 3

Quote about the paper (everything below)
" The scientists discovered that aspirin triggers the production of molecules called resolvins, which help shut off, or “resolve,” the inflammation that can cause chronic disease.

“We found that one resolvin, termed resolvin D3 from the omega-3 fatty acid DHA, persists longer at sites of inflammation,” said senior author Dr. Charles Serhan of Brigham and Women’s Hospital and Harvard Medical School. “This finding suggests that this late resolution phase resolvin D3 might display unique properties in fighting uncontrolled inflammation.”

Researchers confirmed that aspirin treatment triggered the production of a longer acting form of resolvin D3 through a different pathway.

“Aspirin is able to modify an inflammatory enzyme to stop forming molecules that propagate inflammation and instead produce molecules from omega-3 fatty acids, like resolvin D3, that help inflammation to end,” said coauthor Dr. Nicos Petasis of the University of Southern California.

Resolvins are naturally produced by the body from omega-3 fatty acids, but the researchers found that aspirin magnifies their anti-inflammatory effects.

“I regard low-dose aspirin as a tonic and preventive that everyone should consider, particularly those who are at risk for cardiovascular disease,” said University of Arizona professor Dr. Andrew Weil in a statement."

8 Likes
#29

More thoughts on optimizing fish oil and aspirin.

As reported, Attia takes the following supplement stack:

  • EPA | DHA fish oil: 2.5 g | 1 g
  • Vitamin D3: 5000 IU
  • Magnesium: 1 g
  • Vitamin B9: 400 mcg
  • Vitamin B12: 500 mcg.
  • Vitamin B6 (3x per week): 50 mg.
  • Aspirin: 81 mg
  • Pendulum Glucose Control probiotic.

More specifically:

  • EPA | DHA fish oil: 2.5 g | 1 g
  • Aspirin: 81 mg

Seems like a lot of fish oil, but maybe well worth it to mitigate inflammation (as well as all the other good things it can do.

Thoughts?

4 Likes
#30

A gram of magnesium seems high. Typical of Peter though, I wonder what the form is?

2 Likes
#31

He takes SloMag, and Magtien products:

4 Likes
#32

Any feedback on that BTW?

1 Like
#33

The A. Fib issue has me dialing back the fish oil, at least temporarily.

1 Like
#34

Any update on fatty15?

1 Like
#35

Did you ever start this list?

3 Likes
#36

STEMtalk podcast with Dr. Charles Serhan. Charles is a Harvard professor best known for his discovery of specialized pro-resolving mediators. SPMs are molecules that can activate the natural resolution of inflammation and help people avoid anti-inflammatory drugs.

A paper referenced in the podcast
https://www.sciencedirect.com/science/article/pii/S0002944017301359

SPM product mentioned in podcast

IMG_7404
IMG_74041170×1801 145 KB

2 Likes
Physionic Resources
#37

G’day @约瑟夫_拉维尔,

I’ve continued taking Fatty15 from early this year and I have two months stock left which will take me to nine months of continual use at two capsules each day. Last week I pulled my subscription because my recent blood works, although fine, show no tangible benefit nor do I notice any other positive results. That doesn’t mean to say it doesn’t work but it’s expensive and there are other things I think are more promising to add to my minimal stack.

I halved by O3 intake during the Fatty15 use which I’ll now return to. As I’ve mentioned previously, I try to keep my supplements to a minimum. Certainly anything experimental I do one at a time to avoid polluted results.

3 Likes
#38

@JazzMann have you had an omega index test before or after? Thanks.

1 Like
#39

Not yet. Hard to get it done in Aust but I’ll look into it again.

2 Likes
#40

@JazzMann OmegaQuant has a mail order test that’s good.

3 Likes