#203

Me too, but I bought 4 stainless steel screens and did 4 quarts at a time sprouting broccoli, freezing in an ice tray, adding to the smoothie. I didn’t last long, it’s just too much hassle and the flavor is too bad. I felt nothing. Now it looks like it works.Grrr.

#204

image

https://x.com/OraBiomedical/status/1831369415344189730

#TheAcceleration

Next top result is 55% increase in median worm lifespan from Rapamycin Longevity Lab by @Krister_Kauppi with GSK2126458 10µM / Allantoin 50µM, you might want to make a new thread for this. Is it synergistic or just additive?

What other sponsored interventions are interesting, positive or negative?

6 Likes
#205

Carvedilol did very well but did not reach significance: why? (also interesting for nebivolol users @LukeMV, carvedilol is very similar, might be even better) Should I sponsor a lower dose? Higher? It looks like it did very well early in life but then poorly late in life.

Has anyone already sponsored sulforaphane + nilvadipine?

2 Likes
#206

Allantoin by itself did poorly. So it seems to have some unique effect with GSK2126458. I’m wondering if it just helps to preserve skin barrier on very long lived worms? It seems like it mostly is a skin care product.
I don’t think anyone has tested sulforaphane + nilvadipine, I almost did but went for other combos instead.

1 Like
#207

It looks like it does have some CR effects actually… Maybe the GSK just enhanced the absorption of it and the native would need higher dose?

When the researchers tested the effects of allantoin—a botanical extract derived from the comfrey plant—on nematode worms, they found that it mimicked the calorie-restriction process and increased the life span of the worms by 20 percent

“The drug under study was added to 3 mL NGM to give the following final concentration: 10 μm rapamycin, 20 μm geldanamycin, 100 μm LY-294002, 100 μm TSA and 250 μm allantoin”

It looks like the normal 50uM ora dose is just way too low for allantonin to work, although they did find that low dose rapamycin had decent beneficial effect, although it seems allantonin and TSA outperformed it

#208

Hmm… But I doubt the ITP would go about slathering their mice daily with creams. We may need to do a human trial?

#209

Isn’t Allantoin potentially fatal if ingested by humans?

1 Like
#210

I was thinking of a topical application trial.

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#211

It would be nice if they gave a leaderboard with all of the interventions that gave 20%+ life extension so we can see the playing field and be able to propose better combinations. It may be long, but it would be incredibly informative.

1 Like
#212

Its posted above only 9 found so far above 20%. The 10th was 19%

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#213

Sponsored an intervention (Ora Biomedical) — Trichostatin A (TSA). Hopefully we can replicate the 20% life extension even with a lower dose. It seems to have a similar CR function and effect to allantoin so it would probably also pair well with that GSK.

3 Likes
#214

Its quite a strong HDAC inhibitor. I think it probably would have a quite well defined sweet spot where if you go over that point any benefits drop off pretty quickly.

#215

You tested doxycycline and it shortened the lifespan in the early period of the experiment but extended the lifespan in the later period. Carvedilol did the opposite. So I sponsored the combination, hoping to get the best of both worlds (we might also get the worst of both worlds :slight_smile: ).

I’ve also just sponsored Sulforaphane + Nilvadipine.

9 Likes
#216

You’d probably need a ton of it. Allantoin is naturally occurring in some foods, such as true yams. It’s the suspected antidiabetic agent in these, presumably acting similarly to metformin.

I took a few grams a day for a while, but then decided that was stupid.

3 Likes
#217

Great, let’s see how it turns out. I now sponsor Doxycycline HCl + Lithium Citrate and Doxycycline HCl + GSK2126458.

Just a few thoughts:
I note that the first test with Nilvadipine as single agent had short-lived controls. The median was only 12,9 and the Nilvadipine group had a median of 17,9 days,

The results from Sulforaphane as single agent was good, but not as good as one would like. The controls were also in this experiment rather shortlived (14 days)

same goes for the controls in the experiment with GSK2126458 10µM / Allantoin 50µM, the median lifespan was only 14,5 days.

Compared to this the results could be considered better from the experiments with GSK2126458 10µM / Berberine 50µM and GSK2126458 10µM / Metformin HCl 50µM. In those expiriments we got a good result and the controls had a healthy lifespan.

5 Likes
#218

In the MMC research I do like all the well known patent free medicines and chemicals that are tested but I strongly dislike big pharma jumping on this bandwagon with patented analogs of cheap drugs like rapa. or a combi therewith be it in one molecule like GSK2126458 or in multiple molecules.
My hope is afordable increase in healthspan for the masses.

#219

Big pharma isnt sponsoring any of these, its all just a few us members in the forum plus a few other regular people just picking stuff. You are free to pick whatever you want!

5 Likes
#220

Also, whatever big pharma does, we can always count on Indian freedom fighters to make it affordable for us. So I hope big pharma will jump on this bandwagon! The more we screen the better.

6 Likes
#221

Sponsored BEZ235 (Dactolisib)
and GDC-0980 (Apitolisib)

Single interventions

1 Like
#222

Double mtor inhibitors looks like or is there difference? I would think best to use just nest one of each pathway but maybe even 8 different pathways to hit most of the hallmarks of aging…
@adssx when you sponsor custom stuff is it still 100$ per chemical, assuming they have the supplies since those are extra? Hopefully they can get a quantity discount at a certain point…