#82

Hi Keith,

Can you please share what side effects people have experienced so I know what to look out for?

#83

Steve Perry wrote about it in his klotho white paper

“Klotho is a metabolic hormone which makes it as difficult to work with as insulin or thyroid hormone. And most likely equally dangerous in excess amounts.
Between Klotho’s effects on FGF21 and FGF19, the first thing you may notice is feeling full after a small meal.
However, if your Klotho levels get too high, a half sandwich suddenly feels like you ate a big Thanksgiving dinner with a stomach ache to match.
If you keep taking Klotho at this point, then you’ll experience tachycardia (rapid heartbeat) which can be dangerous and take at least an hour to subside.
And if you for some reason don’t take a break and cut the dose after all the above, the next level of side effects is what feels like constriction/tightening of the throat. This probably has something to do with the Klotho/FGF21 effect on the thyroid. This is a scary side effect and if you experience it, you surely will have the good sense to take at least a month off from Klotho and cut the dose substantially.”

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#84

Well if Steve Perry is correct, we’ll expect to see a whole bunch of deceased individuals from the dosing at 500 mcg/dose with Celia Health. I disagree with Steve (may he rest in peace as I have loved watching his old lectures) insulin dosing and thyroid hormone dosing are easy. I do it literally every day for patients.

I think one fundamental problem with klotho is no cost effective and readily accessible way to test it. I’d love if someone finds a reliable option. I’ve not been able to.

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#85

Mystery solved, this product is not klotho protein. It is a small 33 amino acid peptide klotho fragment. Real a-klotho protein is 1,012 aminos. Which explains why the dose is not the same.

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#86

There isnt a problem with sourcing and using real klotho protein, it is affordable and accessible from bucky, canlab etc and has been used for years by many people at the dose of .5pg which has been found to be effective.

#87

@Keith I meant testing levels. Yes I know @Beth was working on finding out what form of klotho they were using, as I’ve been in contact with Bucky and pointed out this product to them that is way cheaper for a similar quantity. BuckyLabs told me that the Alpha Klotho is their product that has the tightest margin on pricing.

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#88

And to add…

The owner of Celia’s comments on facebook convinced me that I would never buy anything from them.

And I happily start my “A” klotho today!!!

@Keith I will try to avoid my throat closing up, so thanks for that list :slight_smile:

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#89

Buckys product needs a complicated dilution sequence, but canlabintl has smaller dose and only needs to be reconstituted so there is no risk of making an error which could inadvertently give you an excess dose, so it is safer. It would cost more in the long run but its a good option to start with and try before you try to tackle the bucky product dilution.

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#90

I really appreciate this great advice!

If I were not a patient of Grant’s, I would definitely need to do this!

Grant has fortunately spoon fed me each detail to make sure nothing goes wrong. And even then, I ran my understanding of the instructions by the engineer husband to double check my math blocked brain :slight_smile:

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#91

Ha, I spoke too soon about having a handle on doing this myself!!

As I was mixing the vial with bacterosteric water to suction it out, the suction pulled the needle off and then 1/3 of it when flying out of the syringe. And now I have no idea what to do with what’s left in the syringe because those calculations for diluting it are all above my pay grade. Just sharing that your idea of getting the smaller vial to begin with was a wise one :).

My walls have klotho but nothing in me yet. :slight_smile:

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#92

DrFraser, since you left a link to Celia I assume it’s a product you feel safe about. Do you know if the Celia Klotho FG is pure alpha Klotho or possibly a peptide fragment? And, if it is a fragment I sure would like to know more about which peptide fragment it is and any effectiveness testing it’s been through. It’s a lot to ask, but if you find the time thanks for any response.

#93

Fyi, it was @jakexb who posted the Celia link.

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#94

No I don’t know it is safe or effective - certainly the mcg dose seems to potentially fit with what would be therapeutic, but it is a klotho fragment, not alpha klotho.

#95

Nice study on a-Klotho levels in Healthy Adults Aged 18–85 Years. Subjects were categorized based on their age as: (i) young (18–34.9 years), (ii) middle-aged (35–54.9 years), and (iii) senior (55–85 years) individuals.

As we age the level drops.

Based on the avg pg/ml and average ml of blood in a human
750pg/ml x 5,000ml blood = 3,750,000pico = 3.75mcg of a-Klotho in circulation.

I agree that adding 0.5pico to a pool of over 3Mpico seems inconsequential.

1 Like
#96

The situation is even worse than this - as it’s not just the 5,000 mL of blood, the other 80 kgs of you has it distributed into tissue … so there is a lot more than 3.75 mcg, but yes, in blood circulation, that is correct.

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#97

From the paper linked above.

klotho-levels

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#98

Unveiling the Emerging Role of Klotho: A Comprehensive Narrative Review of an Anti-aging Factor in Human Fertility

Klotho, an anti-aging protein, plays a vital role in diverse biological functions, such as regulating calcium and vitamin D levels, preventing chronic fibrosis, acting as an antioxidant and anti-inflammatory agent, safeguarding against cardiovascular and neurodegenerative conditions, as well as exerting anti-apoptotic, anti-senescence effects. Additionally, it contributes to metabolic processes associated with diabetes and exhibits anti-cancer properties. This protein is commonly expressed in organs, such as kidneys, brain, pancreas, parathyroid glands, ovaries, and testes. Recent research has highlighted its significance in human fertility. This narrative review provides insight into the involvement of Klotho protein in male and female fertility, as well as its potential role in managing human infertility in the future.

In this study, a search was conducted on literature spanning from November 1997 to June 2024 across multiple databases, including PUBMED, SCOPUS, and Google Scholar, focusing on Klotho proteins. The search utilized keywords, such as “discovery of Klotho proteins,” “Biological functions of Klotho,” “Klotho in female fertility,” “Klotho and PCOS,” “Klotho and cryopreservation,” and “Klotho in male infertility.” Inclusion criteria comprised full-length original or review articles, as well as abstracts, discussing the role of Klotho protein in human fertility, published in English in various peer-reviewed journals. Exclusion criteria involved articles published in languages other than English. Hence, due to its anti-aging characteristics, Klotho protein presents potential roles in male and female fertility and holds promising prospects for reproductive medicine. Further, it holds the potential to become a valuable asset in addressing infertility concerns for both males and females.

Paywalled Review paper:

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#99

Klotho Gene Therapy Extends Lifespan in Mice, Paves Way for Human Trials

A study published in Molecular Therapy reveals that upregulation of secreted Klotho (s-KL) using adeno-associated virus (AAV) gene therapy significantly extends the lifespan of male mice, marking a major advancement in anti-aging science. This research highlights the potential of targeting specific proteins to mitigate age-related decline and improve longevity.

Key Points:

  • Male mice treated with the s-KL AAV at 12 months demonstrated substantial lifespan extension without adverse health effects
  • Enhanced muscle regeneration and structural improvement in treated males were accompanied by reduced fibrosis and better grip strength
  • The therapy also improved neuronal markers and reduced age-related changes in gene expression in the hippocampus

Why It Matters: This research opens up new possibilities for developing anti-aging therapies that could potentially extend human healthspan and lifespan.

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#100

C57Bl6 but interesting nonetheless. Apparently their vector expressed more s-KL in males than in females so this doesn’t mean it won’t work in females.

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#101

The details of the results make me stay away from Klotho.

  • First, if mice receive Klotho at 12 months of age they live longer than the 6 month counterpart.
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  • Second, if you read the adverse effects in female mice, it is horrendous.

I will pass until we know more.

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