#397

I take a lot of pills. 20 60mg pills now 10 12mg and 15 20mg. It was 10x12 10x20 + 10x10 = 1200+120+300=1620mg

2 Likes
#398

Where from? I’m also in Aus

1 Like
#399

I thought you took the powder form…

#400

Rarely. I tried mixed with malt whisky. It put me to sleep.

#401

Efficacy of a Neuroimmune Therapy Including Pineal Methoxyindoles, Angiotensin 1-7, and Endocannabinoids in Cancer, Autoimmune, and Neurodegenerative Diseases

Weird Italian-Argentinian paper:

Patients and Methods: This study evaluates the safety and efficacy of a neuroimmune regimen comprising melatonin (100 mg/day at night), 5-methoxytryptamine (30 mg in the early afternoon), angiotensin 1– 7 (0.5 mg twice daily), and cannabidiol (20 mg twice daily) in 306 patients with NCDs, including advanced cancer, autoimmune diseases, neurodegenerative disorders, depression, and cardiovascular disease.
Results: The neuroimmune regimen successfully halted cancer progression in 68% of cancer patients, who also reported improvements in mood, sleep, and relief from anxiety, pain, and fatigue. In patients with autoimmune diseases, the treatment effectively controlled the disease process, remarkable in cases of multiple sclerosis. Additionally, positive outcomes were observed in patients with Parkinson’s disease, Alzheimer’s disease, and depression.

1 Like
#402

On fertility:

Chinese paper, male mice: Melatonin ameliorates RF-EMR-induced reproductive damage by inhibiting ferroptosis through Nrf2 pathway activation 2025

Iranian preprint, female humans: Melatonin treatment results in progression of endometrial thickness in infertile women in IUI cycle 2025: “In general, administration of melatonin for 4 weeks in infertile women undergoing IUI cycle had beneficial effects on endometrial thickness, which was associated with better pregnancy outcome.”

2 Likes
Melatonin and Exercise Restore Myogenesis and Mitochondrial Dynamics Deficits
#403

my “lifetime” supply of melatonin arrived lol. 33333 doses according to this, 92 years at the recommended dose.

2 Likes
#404

Effects of melatonin implants on the rate of pseudopregnancy in high yielding dairy goats 2025

ChatGPT explanation:

  • Pseudopregnancy (PPG) is the accumulation of sterile uterine fluid together with a persistent corpus luteum; it prolongs anoestrus and causes temporary infertility. On commercial dairy farms the condition typically affects 6–30 % of does and may cluster in specific years or management systems
  • A year-long field study on five Portuguese dairy-goat farms (2023-24) shows that giving does one 18-mg slow-release melatonin implant four to six weeks before buck introduction slashed pseudopregnancy (hydrometra) rates from the usual 7–10 % down to <1 %. Compared with natural, untreated cycles the odds of pseudopregnancy fell ~15-fold, and compared with a standard progesterone-CIDR induction protocol they fell ~40-fold. Primiparous does were still the most vulnerable group, but even they benefitted markedly from melatonin. The work suggests that, in high-yielding herds where intensive progesterone programmes are common, swapping those protocols for pre-breeding melatonin could improve fertility, shorten kidding intervals and reduce veterinary interventions.
1 Like
#405

It taste terrible lol. I feel like I’ve to force myself.

1 Like
#406

I had such a tiny amount I couldn’t taste anything. I dipped a spoon in and then shook off as much as I can. Just a bit of dust stuck to it. Already feel it. I’m unsure how to properly dose this.

#407

I took every melatonin study in this thread and fed it into ChatGPT and got it to spit out a summary of all of them:

:dna: Core Biological Roles of Melatonin

1. Circadian Regulation

  • Melatonin is primarily secreted by the pineal gland and plays a key role in synchronizing circadian rhythms.
  • It follows a light-dependent cycle, peaking at night and signaling darkness to the body, thereby promoting sleep and regulating hormonal cycles (including cortisol and reproductive hormones).

2. Mitochondrial Health & Energy Metabolism

  • Melatonin is synthesized not only in the pineal gland but also in mitochondria.
  • Studies show it enhances mitochondrial function by:
    • Promoting ATP production.
    • Reducing mitochondrial oxidative stress.
    • Improving mitochondrial calcium homeostasis.
  • It can stimulate muscle fiber conversion (from glycolytic to oxidative) in diabetic models—mimicking some effects of endurance exercise.

3. Antioxidant & Free Radical Scavenging

  • Melatonin directly scavenges reactive oxygen and nitrogen species.
  • It also upregulates endogenous antioxidant enzymes (e.g., SOD, glutathione peroxidase).
  • Unique among antioxidants, it crosses the blood-brain barrier and concentrates in mitochondria and nuclei.

:brain: Neuroprotection & Brain Health

4. Alzheimer’s & Parkinson’s Disease

  • Melatonin reduces tau hyperphosphorylation and Aβ accumulation in animal models.
  • Enhances glymphatic clearance of metabolic waste, possibly reducing risk of neurodegeneration.
  • Supports mitochondrial integrity in neurons and may help counter age-related metabolic shifts in brain cells.

5. Cognitive Function & Memory

  • Improves long-term memory formation in rodents via modulation of protein phosphorylation.
  • Enhances glymphatic function post-intracerebral hemorrhage, resulting in improved cognitive recovery.

:person_in_lotus_position: Sleep and Chronobiology

6. Sleep Quality

  • Meta-analyses show optimal efficacy for:
    • 3–4 mg taken ~3 hours before bedtime.
    • Helps reduce sleep onset latency and increase total sleep time.
  • Especially effective in older adults and those with insomnia or circadian rhythm disorders.

7. Jet Lag, Shift Work, and Chronotherapy

  • Well-suited for shifting circadian phase in cases of jet lag and shift work.
  • Has been studied as a tool in chronotherapy to align medication administration with biological rhythms.

:heartbeat: Cardiovascular & Metabolic Health

8. Blood Pressure Regulation

  • Reduces nocturnal blood pressure (more in “non-dippers”), possibly via improved endothelial function and oxidative stress reduction.

9. Glucose Metabolism and Diabesity

  • In diabetic models:
    • Enhances insulin sensitivity.
    • Supports healthy skeletal muscle function.
    • Promotes metabolic reprogramming of muscle and liver.

:eye: Retinal and Ocular Health

10. Age-Related Macular Degeneration (AMD)

  • Protects retinal pigment epithelial (RPE) cells by:
    • Inhibiting necroptosis.
    • Suppressing NLRP3 inflammasome via the SIRT1/Nrf2 pathway.
  • Preserves retinal structure in AMD models.

:microbe: Immune Modulation

11. Dual Immunoregulation

  • Acts as an immune buffer:
    • Stimulates immunity in immunosuppressed states.
    • Suppresses excessive inflammation during cytokine storms.
  • Reduces IL-6 and TNF-α while promoting IL-10 and T-reg activity.
  • Shown to modulate macrophage activity via NF-κB and AA-NAT upregulation.

12. Applications in Autoimmune and Infectious Disease

  • May help in autoimmune diseases and as an adjunct in viral infections (e.g., COVID-19) by dampening hyperinflammation while preserving immune competence.

:dna: Anti-Aging and Lifespan Extension

13. Lifespan Studies

  • Melatonin extended lifespan in several animal models (e.g., flies, mice, aquatic invertebrates), possibly due to its mitochondrial and antioxidant actions.
  • Highlighted in DrugAge as a candidate longevity agent.

14. Aging Phenotypes

  • Maintains mitochondrial and glymphatic function with age.
  • Prevents pathological metabolic reprogramming in aged neurons.

:test_tube: Cancer and Cell Growth

15. Anticancer Activity

  • Shown to inhibit proliferation in some cancer cell lines.
  • May sensitize tumors to chemotherapy while protecting normal cells from side effects (radioprotective and antioxidant roles).

:warning: Safety, Risks, and Considerations

16. Generally Safe — But Not Always Benign

  • Very high doses (up to 200 mg/day) used in research with minimal side effects reported.
  • Most common mild side effects: vivid dreams, morning grogginess, headaches.

17. Rare Case Reports

  • One case report linked melatonin to ventricular arrhythmias (PVCs), which resolved after stopping supplementation — indicates rare individual susceptibility.

18. Hormonal & Developmental Considerations

  • Low melatonin levels in children with hypothalamic hamartomas were linked to precocious puberty, suggesting a role in reproductive timing.

Summary: The Case for Melatonin

Melatonin is a pleiotropic molecule:

  • Antioxidant, immunomodulator, chronobiotic, and neuroprotective agent.
  • It impacts systems far beyond sleep, including metabolism, immunity, cognition, mitochondrial health, and aging.
  • Therapeutically, it is among the most underrated low-toxicity compounds with broad potential — though rare risks exist and personalization is warranted.
2 Likes
#408

Which tends to demonstrate both the strengths and weaknesses of LLMs. The LLM does not have a model of reality so cannot easily identify what is really important. On the other hand most human beings don’t have the knowledge to analyse the input.

#409

I spoon a 1/4 teaspoon of powder into my mouth, drink some water, swish it around, swallow, and drink a bit more water.

Seems to get it down without too much terrible taste exposure.

1 Like
#411

how many mg is a quarter teaspoon?

#412

I tried to down 1/2 teaspoon… Hoping to increase to 1 teaspoon. (About 1.5g)

It will take be a while to tolerate it. It doesn’t dissolve in water. Maybe I’ll try evoo.

#413

I estimate 775 mg to 800 mg.

1 Like
#414

Mine dissolves in water enough to swallow, and it’s from two different sources. I put the dose in a small amount of water (less than a shot) stir it well with a teaspoon, leave it for 30 mins then stir again, and it goes down fine. There is usually some residue left that I swill another small amount of water and swallow it down, which also helps with any slight burning sensations. This is about 40 mins before bedtime.

#415

I got 100g from Pure Bulk. I’m not noticing any particularly bad taste. It’s slightly bitter. Maybe I’m just used to eating horrible tasting herbs (tongkat ali for instance).

1 Like
#416

I’ve just bumped up to 1/2 tsp (eat 1500mg - 1600 mg) about 1/2 hour before bed.

1 Like
#417

Still on fertility: The protective effects of melatonin on testis, sperm parameters quality, and in-vitro fertilization in mice following treatment with aflatoxin B1: An experimental study 2025

These findings demonstrated that MLT can compensate for the adverse effects of AFB1 on the quality of testicular tissue, sperm parameters, sperm DNA, and in vitro fertilization outcomes.