#462

I have the liquid version has any one tried to apply the liquid directly to the face with good result ? I cannot swallow pills

#463

It’s not that easy to calculate because the weight of 500 ml depends on the substance’s density. If the substance has a density of 1 g/mL (which is often the case for water), then 500 ml would be 500 grams. But we are not using water here. For other substances, like Rapa mixed with transcutol, the weight may differ. We need a pharmacist to help with calculation :blush:

#464

I used a nail drill and sandered the yellow coating off, with not too much loss. Worked well.

2 Likes
#465

What do you guys think? Any reason why I should not get them? It is from my local pharmacy.

Sirolimus 1.0% Cream/Ointment:15g $69.95, 30g $109.95, 60g $189.95 (AUD). Divide by 1.5 to get USD.

From what I can see I can’t make the 60g bottle without at least $180usd of rapamycin tablets.

Any disadvantage for using cream or ointment? I should ask for cream isn’t? I get more with cream because of the lighter density.

Is there a shelf life?

1 Like
#466

So I finally thought I may want to make a Rapa cream - just couldn’t find the motivation to do so the past years. Then I read this post of Rapadmin. Has this ever been discussed, as it seems like a decent concern to me? I haven’t been able to find more research on this, but admittedly thus far I’ve only done a quick search. I wouldn’t look forward to wasting time making such a cream, only to break down the ECM.

Source: Rapadmin.
“But there is also research that suggests rapamycin may not be that good for the skin, increasing inflammatory markers (which is not a good sign), and triggering a breakdown of the extracellular matrix:”

"Rapamycin is a long studied molecule affecting mTOR/nutrient signaling and has recently been shown to decrease P16 levels of aging skin21, therefore it was chosen as a positive control of senotherapeutic effect in aging skin models. " … “Rapamycin induced a significant increase in P16 expression, a trend towards increased expression of inflammatory markers (IL6 and IL8), and a significant decrease in Keratin 1 gene expression levels (Fig. 3B). In the dermis, peptide 14 treatment promoted a significant reduction in B2M gene expression, a pro-aging factor, as well as in the expression ofIL8. Rapamycin treatment induced no significant changes in these markers and increased Matrix Metalloproteinase-1 (MMP1) gene expression, indicative of breakdown of the extracellular matrix (Fig. 3C).”

Source:
https://www.biorxiv.org/…/10.1101/2020.10.30.362822v2.full

#467

Yes - I still wonder about that statement from the OneSkin team. The caveats are that they only tested a single dose level of rapamycin (and were making no attempts to optimize the performance of rapamycin in skin aging), they were only using it as a “control” to compare their own compound against. But never-the-less, some pros and cons given what we’ve seen in the research.

My approach is still to use pulsed rapamycin cream - 3 or 4 months on, then off for a few months. If anything is going south (a negative impact on my skin) I should notice it.

We don’t know how important these specific Interleukins are in skin aging. Perhaps P16 is more important than the IL6 and IL8).

We also know that IL-17 seems to be important: IL-17 蛋白在皮肤衰老中发挥重要作用，FDA 批准的疗法可用

4 Likes
#468

Thanks! Have you, or any other members, observed any changes in your skin at all, after using Rapamycin cream? Do you add a preservative, on another note, in your transcutol-Rapamycin cream?

#469

So, I’ve been using the Rapa cream nearly 6 weeks 1-2 times per day, and I find it very drying. Up to the point that it is causing redness that is lasting days. I had to stop using it for a few days.

I assume it’s the transcutol causing that, after going over some studies that suggest it may increase transepidermal water loss. I only use 5% transcutol. (And 0.2% Rapa). (A company apparently selling Rapa cream mentions dryness can sometimes occur but that it will disappear after 1 week. That’s definitely not the case for me Topical Rapamycin - #15 by BandolsMom - Rapamycin - Healthspan)

Has anyone else been running into such issues with dryness? I’d reduce the transcutol as I still think that’s probably the culprit, but at 5% it’s already a low concentration. And I’d think I can’t get the rapa to dissolve well enough if I reduce it even further.

Edit: reading through this thread again I’m now wondering if it is the Rapamycin causing the dryness. As others don’t seem to be having any issues with Transcutol in apparently much higher doses… That’s surprising to read. I did use generous amounts of the Rapa cream twice per day, perhaps that is a problem. And I’ve been using an oil base to simplify the process. Perhaps the Cerave cream Rapadmin uses to mix in the Rapa/Transcutol-mixture has ingredients that counteract TEWL more effectively.

#470

What did you mix the rapamycin/transcutol formula with? I have no issues with my CeraVe based cream.

1 Like
#471

Thanks Rapadmin, I use a simple oil as base cream (sweet almond oil). I felt keeping the base cream simple, increases the chances that the Rapa doesn’t oxidise affecting its stability and efficacy. I also don’t doubt the efficacy of the Rapa, but the dryness makes it far from ideal. I’ll need to use it (far) less often, and probably use an occlusive cream or something on top.

I was using a rather thick ‘layer’ of it twice per day - going to tone that down also. I’ll let the Rapa cream sink in for 30mins, then use an occlusive to seal. I’ll see if that avoids this dryness/redness.

1 Like
#472

Much higher proportion of transcutol here (~30%) and no cream/oil. I haven’t noticed drying in particular in the areas I use it.

3 Likes
#473

Thanks so much for sharing, Sol. If you don’t mind me asking: how long have you been using Rapa cream, and have you noticed any effects at all?
The transcutol may indeed not be the culprit after all.

#474

This sounds like an attempt to sell their product. I would love to see long term study of rapamycin skin cream optimized for absorption and performance. For 2+ years, I’ve used my own rapamycin skin cream based on recipes discussed in this forum, with no ill effects and probably some minor good effects.

#475

A year. Alas, no clear results, not for pigmentation, skin quality, nor toenail fungus. These things take time, so I’ll continue, but I was hoping for faster and clearer outcomes.

1 Like
#476

Thanks so much for sharing Sol. That’s a pity to hear! I had my hopes up too high, I think :slightly_smiling_face:. That study did look so promising, but it was only a small trial of course.

#477

Don’t give up, @Pat25 . There are a lot of variables in skin issues, types, and formulas. I adjust proportions of rapa, of transcutol, how often I use it, etc. All our experiments are part of our collective growing body of knowledge.

2 Likes
#478

Thanks Sol, appreciate it. I hope you will still see an improvement!

1 Like
#479

Brett_Miller, it’s been a while since your $800 order of 30 grams of sirolimus powder from JK Chemicals. Did the order arrive in a reasonable amount of time. I ask because JK Chemicals does not have good ratings on the IndiaMart.com website. Thanks, Jay

1 Like
#480

The order did arrive but the process was bumpy and painful, required patience and an understanding of the loosey goosey Indian approach to things (my 8 trips there helped me to understand that yes means kinda yes, tomorrow means a few weeks)…They got the address wrong and did not fix it. Whole process like 6 weeks. I will order from them again, though, to an Indian address this time…

2 Likes
#481

Curious, do you have any results to report?