Anyone trying something a little more edgy?

[RapAdmin]

Thanks for all the detailed information. I have put Dans account on silence for a few months because i feel hes spreading too much misinformation and that he is too reckless in his use of drugs and in his research. I am considering whether to ban him entirely.

He is welcome to read and learn.

[O_o]

Ah, yes, my bad AnUser. I misunderstood what you were saying.
Indeed, most would prefer routes without having to jab a needle into yourself. Self injections are hardcore.

@Agetron

To clarify…I took a Labcorp blood test for trough then took dose. Which was .7
Retested with Labcorp 1 1/2 to 2 hours later for my t-max. 38.2

I see now, it’ a c-max value that Bicep was referencing and not a percentage. That makes a lot more sense now. Achieving 7x of your c-max is a lot different from 7x the percent bioavailabilty. My bad, Agetron.

@Bicep

There is a misunderstanding here about the multiplier, the effective dose, and the blood level.

To explain what is happening here, I’ll have to show this chart:

The t-max is simply the time after a dose at which the concentration in blood is at its highest. The value he gave, being 38.2 ng/mL, is the c-max, or the maximum concentration in blood at the t-max.

When you stated an increase in the percent bioavailability, I thought you were talking about the AUC - area under the curve. This is typically what biochemists refer to when saying “percent bioavailability”. That was a misunderstanding on my part when you stated a percentage.

You see, if you derive the area under the curve you can calculate the total amount of drug that was actually absorbed into the bloodstream. So, having a 7x increase in the c-max doesn’t mean you increased the bioavailability by 7x. In order to determine that accurately, you need a LOT more data points in order to fit a curve and derive the AUC.

Now, that is not only expensive and tedious to do, but also quite difficult. I’m surprised that Agetron has a PCP that allowed him to do back-to-back blood draws like that, pretty cool! But I think even his PCP would balk at the idea of taking 12 or more blood draws spread across a single day. So, it’s better to simply look at the peak and trough level as a rough estimate of the increase in bioavailability. You can only plot a triangle on the chart, but it still gives you an idea of how much more you’re getting out of each dose.

@RapAdmin

I have put Dans account on silence for a few months because i feel hes spreading too much misinformation and that he is too reckless in his use of drugs and in his research.

That sounds like a reasonable action. Hopefully when he returns, he will have a different perspective on his drug use. I genuinely wish him well, but I’m just not sure I can ever convince him to change his habits.

[AnUser]

@O_o

Ah, yes, my bad AnUser. I misunderstood what you were saying.
Indeed, most would prefer routes without having to jab a needle into yourself. Self injections are hardcore.

It’s not your bad, but that what I was meant and it wasn’t totally clear.
I wouldn’t be surprised if Dan was on some kind of drug while writing what he did. Or a manic episode of some sort, based on the writing style.

[Rayk]

I have a small quantity (1 gram) of LGD4033 in my basement. I have not ingested any of it and I may never do so. There are serious risks with ingesting a powder of dubious origin. The powder came with a certificate from a US lab, but anyone can get certificates printed. I would never have considered taking Rapamycin imported from India if I had not read the positive experiences of users on this forum. Lgd4033 is not even a drug at this point–it is considered a research chemical. This compound may reduce prostate bph, and may prevent, or be useful in the treatment of prostate cancer. LGD4033 also shuts down natural testosterone production and may cause heart or liver problems. This is as edgy as I get. I am looking for drugs that might prove very useful that are at risk of being banned.

[DeStrider]

Normal oral Rapamycin absorption is about 10-15%

[约瑟夫]

@O_o

“idea of taking 12 or more blood draws spread across a single day.”

I post last year about performing multiple BT/ what you call back to back.

Coping the testing, like doing GTT

One BD a base, take the rapamycin dose, then one BD 20 min, then 45, then 1 hour, then every hour for 8 hour. Total would be 13BD over 8 hours.{you can do more BD over more/longer time, ever draw cost an additional $66.00] Then you would have an idea on how much is absorbed for that individual using whatever brand of rapamycin.{different brand will show different absorbing rates]

The issue would be the cost,{13 X $66.00] all would be out of pocket. All day at LabCorp with precise times of the BD, either being pierced 13 times or more humane having a catheter installed from the first BD.

Your personalized dosing rate/schedule.

Any self funding volunteer?

Or anyone pick up the cost and I will volunteer?

[John_Hemming]

There are two issues with multiple draws.
a) How much blood it takes. My weekly blood tests probably use up on average 20ml of blood a week. Personally I think there is a need to lose a little blood either through donation or something merely to maintain the iron balance at a reasonable level. Hence that is not an issue. 12 blood draws in a day could be significant, but I don’t think it is as long as it is not every day.
b) How to do the venepuncture.
I think realistically you would not want twelve separate holes. My own venepuncture repairs in a couple of days. You can tell the difference between my right arm which has done about 40 venepunctures in a year and the left which has done one (when the right arm venepuncture went wrong). However, twelve different holes sounds to me like an issue.

There are many things where I would like to see my own metabolic changes. I particularly would like to know what happens to serum Melatonin. These are not necessarily tests it is easy to get done.

Two arms. I think it is obvious which one has had venepuncture 40 times.

[scta123]

I believe they give you just one intravenous cannulation and then draw blood per schedule. It is easy and simple. I went years ago to have some hormones checked every hour for 8 hours and they did it that way with IV cannula.

[约瑟夫]

@John_Hemming

. 12 blood draws in a day could be significant,

FWIW

No, it is not.

I have personally preformed blood infusion {well over 10,000, yes 10,000] on patients. And have supervised many, many more(countless) over the years.

[约瑟夫]

As I stated above;

“more humane having a catheter”

[约瑟夫]

@John_Hemming

You can tell the difference between my right arm which has done about 40 venepunctures in a year and the left which has done one (when the right arm venepuncture went wrong).

FWIW

From your posted photo

The person{phlebotomist] is not that skilled or is just sloppy.

I have preformed a procedure on the same person {a medical doctor] 1 venipuncture with an 18G/1.2mm butterfly winged set, once every 48 hours for over 15 years{adds up to several thousands and the puncher sites do not look that bad.

[John_Hemming]

There were a range. Some were good. Some not so good. One particular one tried with four punctures in each of my adult son’s arms and still did not succeed.

[约瑟夫]

@John_Hemming

One particular one tried with four punctures in each of my adult son’s arms and still did not succeed.

The action’s/attempt procedure speak for themselves

[scta123]

Has anyone any experience with Mucuna pruriens (L-dopa)?

https://examine.com/supplements/mucuna-pruriens/research/

[Bezesk]

Why not use Selegiline?

[argonaut]

“When you take multiple doses over time, there will be some leftover rapamycin in your system from your previous dose. This is what is known as a trough level or therapeutic dose window. So, depending on when Agetron measured his levels, he could have actually been seeing the collective rapamycin in his system being dumped by GFJ at once.”

It’s my understanding that grapefruit juice only enhances absorption of oral doses of rapamycin by its action in the small intestine, not by increasing bioavailability of the rapamycin already in the bloodstream.

[Agetron]

Yes… true. Except per MAC telling me to do a trough first… then dose t-max. I have my low and high measurements.

I don’t have a surplus of rapamycinin within me. My trough last test was .7 close to insignificant. After dose t-max 38.1.

Trough… after a week from dose

T-max taken 2 hours after dose.

[desertshores]

IMO l-dopa is like many supplements. You probably won’t feel any effects if your body is healthy and you don’t need them. Yes, I use Mucuna pruriens occasionally and still have a bottle on the shelf. Sometimes I use it as a mood lifter. It seems to work for me but it may be a placebo effect. It is not recommended for long-term use unless a doctor is prescribing it for Parkenson’s.

[O_o]

@argonaut

It’s my understanding that grapefruit juice only enhances absorption of oral doses of rapamycin by its action in the small intestine, not by increasing bioavailability of the rapamycin already in the bloodstream.

Correct, it inhibits cytochrome enzymes that usually block or break down drugs like rapamycin. I should have clarified and said the collective rapamycin in his intestinal system, as bloodstream rapamycin would be largely unaffected as you have stated. My bad.

Usually there is some residual drug in the small intestine that is bound up by cytochromes or other surfaces/proteins - call this the “latent” drug. When taking GFJ, this can not only increase the absorption of the next dose, but also increase the amount absorbed that was already residing within the intestines. This is especially so for drugs that undergo enterohepatic recycling, but I’m not sure if rapamycin undergoes that phenomenon.

@Agetron

Except per MAC telling me to do a trough first… then dose t-max. I have my low and high measurements.

Order doesn’t particularly matter, if you’re concerned about that. It’ll still give you a triangular plot, just thicker towards the end rather than the beginning.

@Agetron

I don’t have a surplus of rapamycinin within me. My trough last test was .7 close to insignificant. After dose t-max 38.1.

I see, so it’s quite the dramatic jump. Looks as if your body effectively flushes it out entirely between doses. IMO, that seems pretty good since it indicates you’re getting better absorption, but no to the extent that it lingers in any significant amount over the week.

P.S. I hope you didn’t use Snipping Tool or similar photo editors to make that first image of your test results. Refer this article on why.

[argonaut]

If one is dosing weekly, not daily, isn’t the amount of residual rapamycin in the small intestine likely to be negligible - a tiny percentage of the next dose?